PMID- 14602228
OWN - NLM
STAT- MEDLINE
DCOM- 20040813
LR  - 20220331
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 64
IP  - 11
DP  - 2003 Nov
TI  - The 14 bp deletion-insertion polymorphism in the 3' UT region of the HLA-G gene 
      influences HLA-G mRNA stability.
PG  - 1005-10
AB  - Human leukocyte antigen (HLA)-G molecules are generated by an alternative 
      splicing of the primary transcript of the gene and display specialized function 
      in regulating the immune response. Although HLA-G gene polymorphism is low, it 
      may influence levels of protein expression and, in some cases, has been 
      associated with pregnancy diseases. The HLA-G gene exhibits 14 alleles generating 
      six proteins with minor variations and a null allele. HLA-G allelic variants may 
      be also characterized by a 14 bp deletion-insertion polymorphism located in the 
      3' UT region of the HLA-G gene. The presence of the 14 bp insertion is known to 
      generate an additional splice whereby 92 bases are removed from the start of exon 
      8. To analyze the effect of this deletion on the stability of HLA-G mRNAs, we 
      performed actinomycin D treatments on both JEG-3 choriocarcinoma cell line and M8 
      melanoma cell line transfected with HLA-G*010102 allele. We observed that HLA-G 
      mRNAs having the 92-base deletion are more stable than the complete mRNA forms, 
      suggesting that this region may be involved in the mechanisms controlling 
      post-transcriptional regulation of HLA-G molecule associated with allelic 
      variants.
FAU - Rousseau, Philippe
AU  - Rousseau P
AD  - Commissariat a l'Energie Atomique, SRHI, IUH, Hopital St-Louis 1, Avenue Claude 
      Vellefaux, Paris, France.
FAU - Le Discorde, Magali
AU  - Le Discorde M
FAU - Mouillot, Gael
AU  - Mouillot G
FAU - Marcou, Celine
AU  - Marcou C
FAU - Carosella, Edgardo D
AU  - Carosella ED
FAU - Moreau, Philippe
AU  - Moreau P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *3' Untranslated Regions
MH  - Alleles
MH  - Alternative Splicing
MH  - Cell Line
MH  - Female
MH  - Genetic Variation
MH  - HLA Antigens/*genetics
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - *Polymorphism, Genetic
MH  - Pregnancy
MH  - Promoter Regions, Genetic
MH  - *RNA Stability
MH  - RNA, Messenger/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Deletion
MH  - Transcription, Genetic
MH  - Transfection
MH  - Trophoblasts/chemistry/metabolism
EDAT- 2003/11/07 05:00
MHDA- 2004/08/17 10:00
CRDT- 2003/11/07 05:00
PHST- 2003/11/07 05:00 [pubmed]
PHST- 2004/08/17 10:00 [medline]
PHST- 2003/11/07 05:00 [entrez]
AID - S0198885903005354 [pii]
AID - 10.1016/j.humimm.2003.08.347 [doi]
PST - ppublish
SO  - Hum Immunol. 2003 Nov;64(11):1005-10. doi: 10.1016/j.humimm.2003.08.347.