PMID- 14602233
OWN - NLM
STAT- MEDLINE
DCOM- 20040813
LR  - 20201222
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 64
IP  - 11
DP  - 2003 Nov
TI  - Expression of HLA-G in human cornea, an immune-privileged tissue.
PG  - 1039-44
AB  - Human leukocyte antigen (HLA)-G retains the capacity to modulate immune 
      responses, favoring the establishment of tolerance in solid-tissue 
      allotransplants. To better understand the mechanisms that promote corneal 
      allograft survival, we investigated whether HLA-G was an immunoregulatory factor 
      involved in corneal immunology. We therefore sought HLA-G expression in corneal 
      tissues. Corneal transplantation consists in replacing the center of a diseased 
      cornea with normal corneal tissue. Two corneal parts are not used in such 
      surgery: diseased central corneal tissue and peripheral normal cornea. For this 
      study, we used healthy corneas obtained from deceased donors and diseased corneas 
      obtained from patients with pseudophakic bullous keratopathy or keratoconus who 
      had undergone corneal transplantation. Immunohistochemical analysis carried out 
      on the cryopreserved corneas showed a positive immunohistochemical staining with 
      anti-HLA-G, anti-HLA-A, -B, and -C, and anti-HLA class I monoclonal antibodies. 
      Staining was obtained for keratocytes, epithelial cells, and endothelial cells 
      from both healthy and pathologic human corneas, revealing the presence of HLA 
      class I proteins, including HLA-G. HLA-G transcripts were detected in normal 
      cornea by reverse transcriptase-polymerase chain reaction with a classical 
      pattern of alternative splicing. The detection of HLA-G protein in adult corneas 
      leads to the conclusion that this protein may contribute to the maintenance of 
      the privileged immune status of cornea.
FAU - Le Discorde, Magali
AU  - Le Discorde M
AD  - Service de Recherches en Hemato-Immunologie, CEA-DSV-DRM, Institut d'Hematologie, 
      Hopital Saint-Louis, Paris, France. teyssier@dsvidf.cea.fr
FAU - Moreau, Philippe
AU  - Moreau P
FAU - Sabatier, Patrick
AU  - Sabatier P
FAU - Legeais, Jean-Marc
AU  - Legeais JM
FAU - Carosella, Edgardo D
AU  - Carosella ED
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Adult
MH  - Cornea/cytology/*immunology
MH  - Corneal Diseases/*immunology
MH  - Corneal Transplantation
MH  - Fluorescent Antibody Technique
MH  - HLA Antigens/*analysis/genetics/immunology
MH  - HLA-A Antigens/analysis
MH  - HLA-B Antigens/immunology
MH  - HLA-C Antigens/analysis
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*analysis/genetics/immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratoconus/*immunology
MH  - Pseudophakia/*immunology
MH  - RNA/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/11/07 05:00
MHDA- 2004/08/17 10:00
CRDT- 2003/11/07 05:00
PHST- 2003/11/07 05:00 [pubmed]
PHST- 2004/08/17 10:00 [medline]
PHST- 2003/11/07 05:00 [entrez]
AID - S0198885903005330 [pii]
AID - 10.1016/j.humimm.2003.08.346 [doi]
PST - ppublish
SO  - Hum Immunol. 2003 Nov;64(11):1039-44. doi: 10.1016/j.humimm.2003.08.346.