PMID- 14602574
OWN - NLM
STAT- MEDLINE
DCOM- 20040122
LR  - 20181113
IS  - 1355-0284 (Print)
IS  - 1355-0284 (Linking)
VI  - 9
IP  - 6
DP  - 2003 Dec
TI  - Cloning and initial characterization of an alternatively spliced transcript 
      encoded by the bovine herpes virus 1 latency-related gene.
PG  - 612-22
AB  - Bovine herpesvirus 1 (BHV-1) establishes latency in trigeminal ganglionic sensory 
      neurons of infected cattle. The latency-related (LR) RNA is the only abundantly 
      expressed viral transcript in sensory neurons of latently infected calves. 
      Wild-type expression of LR gene products is required for the latency-reactivation 
      cycle in calves. LR RNA is alternatively spliced in trigeminal ganglia (TG) after 
      infection of calves, suggesting that these alternatively spliced transcripts 
      encode novel factors that regulate specific steps during latency. To begin 
      testing whether these alternatively spliced transcripts have novel functions, the 
      authors cloned a full-length cDNA identified in TG of calves at 7 days post 
      infection (dpi) and compared the functions of this cDNA to the intact LR gene. As 
      a result of splicing, the 7 dpi cDNA contains a novel open reading (ORF) 
      comprised of OFR-2 fused to ORF-1. Overexpression of the 7 dpi cDNA inhibited the 
      BHV-1 immediate-early transcription unit 1 (IEtu1) promoter and the herpes 
      simplex virus type 1 ICP0 promoter. Conversely, the 7 dpi cDNA stimulated the LR 
      promoter in transiently transfected cells. A plasmid containing the LR gene had 
      little effect on IEtu1 or LR promoter activity, indicating that the 7 dpi cDNA 
      has novel functions.
FAU - Devireddy, Laxminarayana R
AU  - Devireddy LR
AD  - Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, 
      University of Nebraska-Lincoln, Lincoln, Nebraska 68583, USA.
FAU - Zhang, Yange
AU  - Zhang Y
FAU - Jones, Clinton J
AU  - Jones CJ
LA  - eng
GR  - 1P20 RR15635/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
RN  - 0 (Recombinant Proteins)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - *Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - Chloramphenicol O-Acetyltransferase/genetics/metabolism
MH  - Cloning, Molecular
MH  - Herpesvirus 1, Bovine/*genetics
MH  - Humans
MH  - Mice
MH  - Open Reading Frames
MH  - Recombinant Proteins/metabolism
MH  - Restriction Mapping
MH  - *Transcription, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Virus Latency/*genetics
EDAT- 2003/11/07 05:00
MHDA- 2004/01/24 05:00
CRDT- 2003/11/07 05:00
PHST- 2003/11/07 05:00 [pubmed]
PHST- 2004/01/24 05:00 [medline]
PHST- 2003/11/07 05:00 [entrez]
AID - 84B0W9K75LBYUBP3 [pii]
AID - 10.1080/13550280390247542 [doi]
PST - ppublish
SO  - J Neurovirol. 2003 Dec;9(6):612-22. doi: 10.1080/13550280390247542.