PMID- 14602621
OWN - NLM
STAT- MEDLINE
DCOM- 20040220
LR  - 20220331
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 69
IP  - 11
DP  - 2003 Nov
TI  - Characterization of a Lactococcus lactis strain that secretes a major epitope of 
      bovine beta-lactoglobulin and evaluation of its immunogenicity in mice.
PG  - 6620-7
AB  - Bovine beta-lactoglobulin (Blg) is one of the major cow's milk allergens. Peptide 
      41-60 of Blg (Blg41-60) was described as a murine T-cell determinant and a 
      murine, rat, and human immunoglobulin E (IgE) epitope. The aim of this study was 
      the expression of Blg41-60 as a fusion protein in the food-grade bacterium 
      Lactococcus lactis and the characterization of its immunogenicity in mice. We 
      constructed a recombinant strain of L. lactis capable of inducible production and 
      secretion of Blg41-60::Nuc, a fusion protein between Blg41-60 and the mature part 
      of the staphylococcal nuclease (Nuc). The highest production yield of 
      Blg41-60::Nuc (32.5 mg/liter) was reached 4 h after induction. At this time, up 
      to 75% of Blg41-60::Nuc was secreted. When monoclonal antibodies specific for 
      Blg41-60 were used, purified Blg41-60::Nuc and synthetic Blg41-60 exhibited very 
      similar immunoreactivities. Subcutaneous coadministration of purified 
      Blg41-60::Nuc and killed nonrecombinant L. lactis resulted in the induction of 
      specific anti-Blg41-60 IgG2a and IgG1. The IgG1/IgG2a ratio and the lack of 
      specific IgE suggest a Th1-type immune response, i.e., a nonallergic response. 
      Similar administrations of the killed Blg41-60::Nuc-producing L. lactis strain 
      did not elicit a specific immune response, whereas a transitory mucosal 
      IgA-specific immune response was induced in mice after oral administration of the 
      live Blg41-60::Nuc-producing L. lactis strain.
FAU - Chatel, Jean-Marc
AU  - Chatel JM
AD  - Unite d'Immuno-Allergie Alimentaire, INRA/CEA, CE de Saclay, DRM-SPI, 91191 
      Gif-sur-Yvette, France.
FAU - Nouaille, Sebastien
AU  - Nouaille S
FAU - Adel-Patient, Karine
AU  - Adel-Patient K
FAU - Le Loir, Yves
AU  - Le Loir Y
FAU - Boe, Herman
AU  - Boe H
FAU - Gruss, Alexandra
AU  - Gruss A
FAU - Wal, Jean-Michel
AU  - Wal JM
FAU - Langella, Philippe
AU  - Langella P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin A, Secretory)
RN  - 0 (Lactoglobulins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Monoclonal/biosynthesis/immunology
MH  - Cattle
MH  - Epitopes/immunology/*metabolism
MH  - Immunity, Mucosal
MH  - Immunoglobulin A, Secretory/biosynthesis
MH  - Injections, Subcutaneous
MH  - Lactococcus lactis/genetics/*immunology
MH  - Lactoglobulins/genetics/immunology/*metabolism
MH  - Mice
MH  - Micrococcal Nuclease/genetics/immunology/*metabolism
MH  - Recombinant Fusion Proteins/immunology/*metabolism
PMC - PMC262270
EDAT- 2003/11/07 05:00
MHDA- 2004/02/21 05:00
PMCR- 2003/11/01
CRDT- 2003/11/07 05:00
PHST- 2003/11/07 05:00 [pubmed]
PHST- 2004/02/21 05:00 [medline]
PHST- 2003/11/07 05:00 [entrez]
PHST- 2003/11/01 00:00 [pmc-release]
AID - 0579 [pii]
AID - 10.1128/AEM.69.11.6620-6627.2003 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2003 Nov;69(11):6620-7. doi: 
      10.1128/AEM.69.11.6620-6627.2003.