PMID- 14603437
OWN - NLM
STAT- MEDLINE
DCOM- 20040106
LR  - 20071114
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 39
IP  - 1
DP  - 2004 Jan
TI  - Frequent amplification and rearrangement of chromosomal bands 6p12-p21 and 
      17p11.2 in osteosarcoma.
PG  - 11-21
AB  - Osteosarcoma (OS) is a highly malignant bone neoplasm of children and young 
      adults. It is characterized by chaotic karyotypes with complex marker 
      chromosomes. We applied a combination of molecular cytogenetic techniques 
      including comparative genomic hybridization (CGH), spectral karyotyping (SKY), 
      and fluorescence in situ hybridization (FISH) to decipher the chromosomal 
      complexity in a panel of 25 tumors. Combined SKY and G-banding analysis 
      identified several novel recurrent breakpoint clusters and 9 nonrecurrent 
      reciprocal translocations. CGH identified several recurrent chromosomal losses 
      including 2q, 3p, 9, 10p, 12q, 13q, 14q, 15q, 16, 17p, and 18q, gains including 
      Xp, Xq, 5q, 6p, 8q, 17p, and 20q, and high-level chromosomal amplifications at 
      Xp11.2, 1q21-q22, 4p11, 4q12, 5p15, 6p12.1, 8q13, 8q23, 10q11, 10q22, 11q13, 
      11q23, 12q13-q14, 13q21-q34, 16q22, 17p11.2, 17q21-q22, 18q22, 20p11.2, and 
      20q12. Frequent amplification and rearrangement involving chromosomal bands at 
      6p12-p21 and 17p11.2 were found in 28% and 32% of cases, respectively. In an 
      attempt to identify the genes involved in these amplicons, we used three 
      nonoverlapping BAC clones contained within each amplicon as probes for FISH 
      analysis, leading to a more detailed characterization and quantification of the 
      6p and 17p amplicons.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Lau, Ching C
AU  - Lau CC
AD  - Department of Pediatrics, Texas Children's Cancer Center, Baylor College of 
      Medicine, Houston, Texas 77030, USA. clau@txccc.org
FAU - Harris, Charles P
AU  - Harris CP
FAU - Lu, Xin-Yan
AU  - Lu XY
FAU - Perlaky, Laszlo
AU  - Perlaky L
FAU - Gogineni, Sheila
AU  - Gogineni S
FAU - Chintagumpala, Murali
AU  - Chintagumpala M
FAU - Hicks, John
AU  - Hicks J
FAU - Johnson, Mark E
AU  - Johnson ME
FAU - Davino, Nelson A
AU  - Davino NA
FAU - Huvos, Andrew G
AU  - Huvos AG
FAU - Meyers, Paul A
AU  - Meyers PA
FAU - Healy, John H
AU  - Healy JH
FAU - Gorlick, Richard
AU  - Gorlick R
FAU - Rao, Pulivarthi H
AU  - Rao PH
LA  - eng
GR  - CA88126/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Chromosome Banding
MH  - Chromosome Breakage/genetics
MH  - Chromosome Painting
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Chromosomes, Human, Pair 6/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Gene Dosage
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Male
MH  - Nucleic Acid Hybridization
MH  - Osteosarcoma/*genetics
EDAT- 2003/11/07 05:00
MHDA- 2004/01/07 05:00
CRDT- 2003/11/07 05:00
PHST- 2003/11/07 05:00 [pubmed]
PHST- 2004/01/07 05:00 [medline]
PHST- 2003/11/07 05:00 [entrez]
AID - 10.1002/gcc.10291 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2004 Jan;39(1):11-21. doi: 10.1002/gcc.10291.