PMID- 14603442
OWN - NLM
STAT- MEDLINE
DCOM- 20040106
LR  - 20191210
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 39
IP  - 1
DP  - 2004 Jan
TI  - A nonredundant multicolor bar code as a screening tool for rearrangements in 
      neoplasia.
PG  - 59-70
AB  - A chromosome bar code describes the colored pattern of chromosome segments and is 
      derived by multicolor fluorescence in situ hybridization (FISH) of defined 
      molecular probes. Published approaches to the simultaneous differentiation of 
      whole karyotypes with bar codes have not allowed the unequivocal identification 
      of all chromosome segments because of color redundancy of the patterns from a 
      multitude of identically colored segments. Here, we present a chromosome bar code 
      approach in which the problem of color redundancy has been overcome. It allows 
      the detailed description of translocations, including breakpoints as well as 
      intrachromosomal rearrangements in the karyotype of tumor cells. The resolution 
      of discernable bars was increased to 100 bars per haploid chromosome set by 
      including human chromosome-specific probes and more well-defined subregional 
      probes such as chromosome arm- and segment-specific probes. Technically, no 
      limitation to further increase in the resolution of the pattern became apparent. 
      The approach was validated by the analysis of four established tumor cell lines 
      widely used as models in cell biology, revealing numerous inter- and 
      intrachromosomal rearrangements. Chromosome bar coding as presented here may 
      provide further useful information for the subregional assignment of chromosomal 
      breakpoints in complex chromosome aberrations, as found in various neoplasms that 
      cannot be obtained by chromosome painting or classical banding techniques alone.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Muller, Stefan
AU  - Muller S
AD  - Institute of Anthropology and Human Genetics, Department of Biology II, 
      Ludwig-Maximilians-University, Munich, Germany.
FAU - Eder, Verena
AU  - Eder V
FAU - Wienberg, Johannes
AU  - Wienberg J
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chromosome Aberrations
MH  - Colonic Neoplasms/genetics/pathology
MH  - *DNA Probes
MH  - Fluorescent Dyes
MH  - Gene Rearrangement/*genetics
MH  - Genetic Testing/*methods
MH  - Humans
MH  - Jurkat Cells
MH  - Leukemia-Lymphoma, Adult T-Cell/genetics/pathology
MH  - Lymphocytes/chemistry/cytology/pathology
MH  - Male
MH  - Melanoma/chemistry/genetics/pathology
MH  - Neoplasms/chemistry/*genetics
EDAT- 2003/11/07 05:00
MHDA- 2004/01/07 05:00
CRDT- 2003/11/07 05:00
PHST- 2003/11/07 05:00 [pubmed]
PHST- 2004/01/07 05:00 [medline]
PHST- 2003/11/07 05:00 [entrez]
AID - 10.1002/gcc.10301 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2004 Jan;39(1):59-70. doi: 10.1002/gcc.10301.