PMID- 14604286
OWN - NLM
STAT- MEDLINE
DCOM- 20040220
LR  - 20191026
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 78
IP  - 3
DP  - 2003 Oct
TI  - Induction of umbilical cord blood mesenchymal stem cells into neuron-like cells 
      in vitro.
PG  - 256-61
AB  - Mesenchymal stem cells (MSCs) in human umbilical cord blood are multipotent stem 
      cells that differ from hematopoietic stem cells. They can differentiate in vitro 
      into mesenchymal cells such as osteoblasts and adipocytes. However, 
      differentiation into nonmesenchymal cells has not been demonstrated. Here, we 
      report the isolation, purification, expansion, and differentiation of human 
      umbilical cord blood MSCs into neurocytes in vitro. Cord blood samples were 
      allowed to drain from the end of the cord into glass bottles with 20 U/mL 
      preservative-free heparin. MSCs were isolated from human umbilical cord blood, 
      purified, and expanded in Mesencult medium. Surface antigens of MSCs were 
      analyzed by fluorescence-activated cell sorting (FACS). MSC passages 2,5, and 8 
      were induced to differentiate into neuron-like cells. Neurofilament (NF) and 
      neuron-specific enolase (NSE) were detected by immunohistochemistry staining. 
      Special Nissl bodies were observed by histochemical analysis. The results showed 
      that 6.6 x 10(5) primary MSCs were expanded for 10 passages to obtain 9.9 x 10(8) 
      MSCs, an increase of approximately 1.5 x 10(3)-fold. FACS results showed that the 
      MSCs did not express antigens CD34, CD11a, and CD11b and expressed CD29 and CD71, 
      an expression pattern identical to that of human bone marrow-derived MSCs. 
      Induction results indicated that approximately 70% of the cells exhibited a 
      typical neuron-like phenotype. Immunohistochemistry staining suggested that 
      induced MSCs of different passages expressed NF and NSE. Special Nissl bodies 
      were obvious in the neuron-like cells. These results suggest that MSCs in human 
      umbilical cord blood are capable of differentiating into neuron-like cells in 
      vitro.
FAU - Hou, Lingling
AU  - Hou L
AD  - Beijing Institute of Transfusion Medicine, Beijing, China.
FAU - Cao, Hua
AU  - Cao H
FAU - Wang, Dongmei
AU  - Wang D
FAU - Wei, Guorong
AU  - Wei G
FAU - Bai, Cixian
AU  - Bai C
FAU - Zhang, Yong
AU  - Zhang Y
FAU - Pei, Xuetao
AU  - Pei X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Ion Channels)
SB  - IM
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation
MH  - Cell Lineage/*drug effects
MH  - Cell Separation
MH  - Cell Size
MH  - Electrophysiology
MH  - Fetal Blood/*cytology
MH  - Humans
MH  - Immunophenotyping
MH  - Ion Channels
MH  - Mesenchymal Stem Cells/*cytology
MH  - Neurons/*cytology/physiology/ultrastructure
MH  - Nissl Bodies
EDAT- 2003/11/08 05:00
MHDA- 2004/02/21 05:00
CRDT- 2003/11/08 05:00
PHST- 2003/11/08 05:00 [pubmed]
PHST- 2004/02/21 05:00 [medline]
PHST- 2003/11/08 05:00 [entrez]
AID - 10.1007/BF02983804 [doi]
PST - ppublish
SO  - Int J Hematol. 2003 Oct;78(3):256-61. doi: 10.1007/BF02983804.