PMID- 14604680
OWN - NLM
STAT- MEDLINE
DCOM- 20040730
LR  - 20190901
IS  - 0968-0896 (Print)
IS  - 0968-0896 (Linking)
VI  - 11
IP  - 23
DP  - 2003 Nov 17
TI  - Relationship between protective effect of xanthone on endothelial cells and 
      endogenous nitric oxide synthase inhibitors.
PG  - 5171-7
AB  - 1,3,5,6-tetrahydroxyxanthone was synthesized. The relationship between protective 
      effect of xanthone on endothelial cells and endogenous nitric oxide synthase 
      inhibitors was investigated. Endothelial cells were treated with ox-LDL (100 
      microg/mL) for 48 h. Adhesion of monocytes to endothelial cells and release of 
      lactate dehydrogenase (LDH) was determined. Levels of tumor necrosis factor-alpha 
      (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1), nitric oxide (NO) and 
      asymmetric dimethylarginine (ADMA, an endogenous inhibitor of nitric oxide 
      synthase) in conditioned medium and activity of dimethylarginine 
      dimethylaminohydrolase (DDAH) in endothelial cells were measured. Incubation of 
      endothelial cells with ox-LDL (100 microg/mL) for 48 h markedly enhanced the 
      adhesion of monocytes to endothelial cells, increased the release of LDH, the 
      levels of TNF-alpha, MCP-1 and ADMA, and decreased the content of NO and the 
      activity of DDAH. Xanthone (1,3,5,6-tetrahydroxyxanthone) (1, 3 or 10 micromol/L) 
      significantly inhibited the increased adhesion of monocytes to endothelial cells 
      and attenuated the increased levels of LDH, MCP-1 and ADMA induced by ox-LDL. 
      Xanthone (1,3,5,6-tetrahydroxyxanthone) (3 or 10 micromol/L) significantly 
      attenuated the increased level of TNF-alpha and decreased level of NO and 
      activity of DDAH by ox-LDL. The present results suggest that xanthone 
      (1,3,5,6-tetrahydroxyxanthone) preserves endothelial cells and inhibits the 
      increased adhesion of monocytes to endothelial cells induced by ox-LDL, and that 
      the protective effect of xanthone (1,3,5,6-tetrahydroxyxanthone) on endothelial 
      cells is related to reduction of ADMA concentration via increase of DDAH 
      activity.
FAU - Jiang, De-Jian
AU  - Jiang DJ
AD  - Department of Pharmacology, School of Pharmaceutical Sciences, Central South 
      University, Changsha 410078, China.
FAU - Hu, Gao-Yun
AU  - Hu GY
FAU - Jiang, Jun-Lin
AU  - Jiang JL
FAU - Xiang, Hong-Lin
AU  - Xiang HL
FAU - Deng, Han-Wu
AU  - Deng HW
FAU - Li, Yuan-Jian
AU  - Li YJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Bioorg Med Chem
JT  - Bioorganic & medicinal chemistry
JID - 9413298
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Xanthones)
RN  - 0 (dimethylarginine)
RN  - 94ZLA3W45F (Arginine)
RN  - 9749WEV0CA (xanthone)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.3.18 (dimethylargininase)
SB  - IM
MH  - Amidohydrolases/metabolism
MH  - Arginine/analogs & derivatives/metabolism
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Endothelium, Vascular/cytology/*drug effects/enzymology/metabolism
MH  - Enzyme Inhibitors/*pharmacology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Monocytes/cytology
MH  - Nitrates/metabolism
MH  - Nitric Oxide Synthase/*antagonists & inhibitors
MH  - Nitrites/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Xanthones/*pharmacology
EDAT- 2003/11/08 05:00
MHDA- 2004/07/31 05:00
CRDT- 2003/11/08 05:00
PHST- 2003/11/08 05:00 [pubmed]
PHST- 2004/07/31 05:00 [medline]
PHST- 2003/11/08 05:00 [entrez]
AID - S0968089603005637 [pii]
AID - 10.1016/j.bmc.2003.08.015 [doi]
PST - ppublish
SO  - Bioorg Med Chem. 2003 Nov 17;11(23):5171-7. doi: 10.1016/j.bmc.2003.08.015.