PMID- 14604807
OWN - NLM
STAT- MEDLINE
DCOM- 20040122
LR  - 20190708
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 317
IP  - 1-2
DP  - 2003 Oct 23
TI  - Highly differentiated and conserved sex chromosome in fish species (Aulopus 
      japonicus: Teleostei, Aulopidae).
PG  - 187-93
AB  - While highly differentiated and long-conserved sex chromosomes such as XY and ZW 
      chromosomes are observed, respectively, in mammalian and avian species, no 
      counterparts to such chromosomes were observed in fish until we reported in the 
      previous study that well-conserved and highly differentiated ZW sex chromosomes 
      existed in the family of Synodontidae. Then, the problem was if the evolutionary 
      history of the fish ZW chromosomes was long enough to be comparable to the 
      mammalian and avian counterparts. To tackle the problem, we had to extend our 
      finding of the fish sex chromosomes further than a family alone. For this 
      purpose, we chose Aulopus japonicus that belonged to one of the related families 
      to Synodontidae. Our cytogenetic and fluorescence in situ hybridization (FISH) 
      analyses have clearly demonstrated that A. japonicus also has ZW chromosomes. We 
      have also found that 5S rDNA clusters are located on the Z and W chromosomes in 
      this species. Using nontranscribed intergenic sequences in the 5S rDNA clusters 
      as PCR primers, we successfully amplified a 6-kb-long female-specific sequence on 
      the W chromosome. The 6-kb-long sequence contained one transposable element and 
      two tRNA sequences. The function of the sequence remains to be studied. Our 
      Southern blot analysis confirmed that the 6-kb sequence was located only on the W 
      chromosome.Therefore, it is now said that highly differentiated ZW chromosomes 
      have been conserved over two fish families. As these families were reported to 
      have been diverged 30-60 million years ago, the fish ZW chromosomes have an 
      evolutionary history corresponding to the history of the families. This is 
      perhaps the first case that fish sex chromosomes are shown to have such a long 
      evolutionary lineage.
FAU - Ota, Kinya
AU  - Ota K
AD  - Center for Information Biology and DNA Data Bank of Japan, National Institute of 
      Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.
FAU - Tateno, Yoshio
AU  - Tateno Y
FAU - Gojobori, Takashi
AU  - Gojobori T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Evolution, Molecular
MH  - Female
MH  - Fishes/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - RNA, Ribosomal, 5S/genetics
MH  - Sex Chromosomes/*genetics
MH  - Sex Factors
EDAT- 2003/11/08 05:00
MHDA- 2004/01/24 05:00
CRDT- 2003/11/08 05:00
PHST- 2003/11/08 05:00 [pubmed]
PHST- 2004/01/24 05:00 [medline]
PHST- 2003/11/08 05:00 [entrez]
AID - S0378111903007029 [pii]
AID - 10.1016/s0378-1119(03)00702-9 [doi]
PST - ppublish
SO  - Gene. 2003 Oct 23;317(1-2):187-93. doi: 10.1016/s0378-1119(03)00702-9.