PMID- 14605445
OWN - NLM
STAT- MEDLINE
DCOM- 20040219
LR  - 20231213
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 47
IP  - 10
DP  - 2003
TI  - Differential expression and signaling of IFN-gamma in the conjunctiva between 
      Lewis and Brown Norway rats.
PG  - 785-96
AB  - Genetic background determines the histological features of experimental 
      immune-mediated blepharoconjunctivitis (EC) in rats, which is a model for human 
      allergic conjunctivitis (AC). A great number of lymphocytes predominate in EC of 
      Lewis rats, while less lymphocytes and more eosinophils are present in that of 
      Brown Norway (BN) rats. Although this difference could be attributed to their 
      systemic Th1/Th2 dominancy, it remains unclear whether some regulatory mechanisms 
      may exist in the inflammatory site in the conjunctiva. Here, we aim to 
      investigate this hypothesis by comparing the expression levels of inflammatory 
      mediators in the conjunctiva between the two strains. EC was induced in Lewis and 
      BN rats by transfer of ovalbumin (OVA)-specific CD4(+) T-cell lines followed by 
      eye drops of OVA as antigen challenge, and then was clinically and histologically 
      evaluated. Reverse-transcription (RT)-PCR was performed to compare the 
      expressions of cytokines and cytokine receptors (Rs) in conjunctivas of both 
      strains of rats either with or without EC. To confirm the biological significance 
      of interferon (IFN)-gamma R expression, phosphorylation of signal transducers and 
      activators of transcription (STAT)-1 was examined in the conjunctivas, followed 
      by subconjunctival injection of IFN-gamma. BN T cells contained interleukin 
      (IL)-4 and IFN-gamma, while Lewis T cells expressed no IL-4. Transfer of those 
      cells induced more severe EC in Lewis rats. RTPCR using naive conjunctivas 
      detected more IL-4, IFN-gamma, and IFN-gamma R beta-chain RNA expression in BN 
      rats. After the EC induction, BN rats expressed significantly higher amounts of 
      IFN-gamma R beta-chain, and upregulation of interferon regulatory factor (IRF)-1 
      was observed. Phosphorylation of STAT-1 was more remarkable in BN rats. The 
      findings demonstrate differential expression of IFN-gamma R and signaling through 
      IFN-gamma in the conjunctiva between the two strains. This may be due to 
      differences in histopathological character between the two strains.
FAU - Fukushima, Atsuki
AU  - Fukushima A
AD  - Laboratory of Immunology, Department of Ophthalmology, Kochi Medical School, 
      Nankoku, Kochi 783-8505, Japan. fukusima@kochi-ms.ac.jp
FAU - Ozaki, Akemi
AU  - Ozaki A
FAU - Fukata, Kazuyo
AU  - Fukata K
FAU - Ueno, Hisayuki
AU  - Ueno H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Interferon)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 0 (Stat1 protein, rat)
RN  - 0 (Trans-Activators)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Blepharitis/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Line
MH  - Conjunctiva/*immunology
MH  - Conjunctivitis, Allergic/*immunology
MH  - DNA-Binding Proteins/metabolism
MH  - Humans
MH  - Interferon-gamma/immunology/*metabolism
MH  - Male
MH  - Ovalbumin/immunology
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred Lew
MH  - Receptors, Interferon/metabolism
MH  - STAT1 Transcription Factor
MH  - *Signal Transduction
MH  - Species Specificity
MH  - Trans-Activators/metabolism
MH  - Interferon gamma Receptor
EDAT- 2003/11/08 05:00
MHDA- 2004/02/20 05:00
CRDT- 2003/11/08 05:00
PHST- 2003/11/08 05:00 [pubmed]
PHST- 2004/02/20 05:00 [medline]
PHST- 2003/11/08 05:00 [entrez]
AID - 10.1111/j.1348-0421.2003.tb03436.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 2003;47(10):785-96. doi: 10.1111/j.1348-0421.2003.tb03436.x.