PMID- 14607005
OWN - NLM
STAT- MEDLINE
DCOM- 20040716
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 27
IP  - 7
DP  - 2003 Oct
TI  - Postmortem identification and quantitation of 
      2,5-dimethoxy-4-n-propylthiophenethylamine using GC-MSD and GC-NPD.
PG  - 493-8
AB  - 2,5-Dimethoxy-4-n-propylthiophenethylamine (2C-T-7) has structural and 
      pharmacodynamic similarities to methylenedioxymethamphetamine (MDMA). This 
      compound was initially identified from a routine screening procedure in 
      postmortem urine from a 20-year-old male that died in a local emergency room 
      after reportedly insufflating 35 mg. This report describes the development of a 
      quantitative method for 2C-T-7. A number of method parameters were studied 
      including internal standard selection, liquid-liquid extraction scheme, and drug 
      stability in preserved refrigerated blood. The adopted method for blood and urine 
      involves the addition of trimethoxyamphetamine (TMA) as internal standard, 
      alkalinization with ammonium hydroxide, and liquid-liquid extraction with 
      n-chlorobutane. To facilitate recovery from liver, a 1:4 aqueous homogenate was 
      pretreated with dilute perchloric acid, centrifuged, and the supernatant was 
      extracted as previously described. In each case, 0.1% hydrochloric acid in 
      methanol was added during the final concentration step to prevent loss of drug 
      caused by evaporation. Samples were analyzed by gas chromatography with 
      nitrogen-phosphorus detection (GC-NPD) and electron ionization GC-mass 
      spectrometry (MS) utilizing selected ion monitoring. For the GC-MS analysis, the 
      characteristic ions monitored for 2C-T-7 were m/z 226, 255, and 183 and for TMA, 
      m/z 182. The limits of detection and quantitation in blood were 6.0 and 15.6 
      ng/mL, respectively, by both GC-NPD and GC-MS. The results from the postmortem 
      case were as follows: heart blood, 57 ng/mL; femoral blood, 100 ng/mL; urine, 
      1120 ng/mL; and liver, 854 ng/g.
FAU - Curtis, Byron
AU  - Curtis B
AD  - University of Oklahoma Health Science Center, Oklahoma City, Oklahoma, USA. 
      chemist@ocmeokc.state.ok.us
FAU - Kemp, Philip
AU  - Kemp P
FAU - Harty, Linda
AU  - Harty L
FAU - Choi, Chai
AU  - Choi C
FAU - Christensen, Dix
AU  - Christensen D
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (2,5-dimethoxy-4-n-propylthiophenethylamine)
RN  - 0 (Phenethylamines)
SB  - IM
MH  - Adult
MH  - Chromatography, Gas
MH  - Fatal Outcome
MH  - Humans
MH  - Male
MH  - Phenethylamines/*analysis/*poisoning
MH  - Postmortem Changes
MH  - Reference Standards
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Substance Abuse Detection/*methods
EDAT- 2003/11/11 05:00
MHDA- 2004/07/17 05:00
CRDT- 2003/11/11 05:00
PHST- 2003/11/11 05:00 [pubmed]
PHST- 2004/07/17 05:00 [medline]
PHST- 2003/11/11 05:00 [entrez]
AID - 10.1093/jat/27.7.493 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2003 Oct;27(7):493-8. doi: 10.1093/jat/27.7.493.