PMID- 14607522
OWN - NLM
STAT- MEDLINE
DCOM- 20040706
LR  - 20210218
IS  - 0891-5849 (Print)
IS  - 0891-5849 (Linking)
VI  - 35
IP  - 10
DP  - 2003 Nov 15
TI  - Proteomic identification of age-dependent protein nitration in rat skeletal 
      muscle.
PG  - 1229-39
AB  - Age-related protein nitration was studied in skeletal muscle of Fisher 344 and 
      Fisher 344/Brown Norway (BN) F1 rats by a proteomic approach. Proteins from young 
      (4 months) and old (24 months) Fisher 344 rats and young (6 months) and old (34 
      months) Fisher 344/BN F1 animals were separated by 2-D gel electrophoresis. 
      Western blot showed an age-related increase in the nitration of a few specific 
      proteins, which were identified by MALDI-TOF MS and ESI-MS/MS. We identified 
      age-dependent apparent nitration of beta-enolase, alpha-fructose aldolase, and 
      creatine kinase, which perform important functions in muscle energy metabolism, 
      suggesting that the nitration of such key proteins can be, in part, responsible 
      for the decline of muscle motor function of the muscle. Furthermore, we have 
      identified the apparent nitration of succinate dehydrogenase, rab GDP 
      dissociation inhibitor beta (GdI-2), triosephosphate isomerase, troponin I, 
      alpha-crystallin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
FAU - Kanski, Jaroslaw
AU  - Kanski J
AD  - Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA.
FAU - Alterman, Michail A
AU  - Alterman MA
FAU - Schoneich, Christian
AU  - Schoneich C
LA  - eng
GR  - 2PO1AG12993/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 14691-52-2 (Peroxynitrous Acid)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.3.2 (Creatine Kinase)
RN  - EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Age Distribution
MH  - Animals
MH  - Blotting, Western
MH  - Creatine Kinase/*metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Energy Metabolism
MH  - Fructose-Bisphosphate Aldolase/*metabolism
MH  - Motor Activity/physiology
MH  - Muscle, Skeletal/enzymology
MH  - Peroxynitrous Acid/*metabolism
MH  - Phosphopyruvate Hydratase/*metabolism
MH  - *Proteomics
MH  - Rats
MH  - Rats, Inbred F344
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Tyrosine/*metabolism
EDAT- 2003/11/11 05:00
MHDA- 2004/07/09 05:00
CRDT- 2003/11/11 05:00
PHST- 2003/11/11 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2003/11/11 05:00 [entrez]
AID - S0891584903005008 [pii]
AID - 10.1016/s0891-5849(03)00500-8 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2003 Nov 15;35(10):1229-39. doi: 
      10.1016/s0891-5849(03)00500-8.