PMID- 14610746
OWN - NLM
STAT- MEDLINE
DCOM- 20040122
LR  - 20211103
IS  - 1008-9292 (Print)
IS  - 1008-9292 (Linking)
VI  - 32
IP  - 5
DP  - 2003 Oct
TI  - [Expression of brain-derived neurotrophic factor at acute inflammatory injury of 
      the brain].
PG  - 433-6
AB  - OBJECTIVE: To investigate the expression of brain-derived neurotrophic factor 
      (BDNF) mRNA and immunoreactivity in experimental acute inflammatory brain injury. 
      METHODS: Ten rats were inoculated with pneumococcus to establish the model of 
      bacterial inflammatory brain injury and other 6 rats were used as normal 
      controls. At 24 h after inoculating, the expression of BDNF mRNA and BDNF protein 
      in brain tissue was detected by in situ hybridization and immunohistochemical 
      methods, respectively. RESULT: The necrosis of neuron in cerebral cortex and 
      hippocampus was observed after infection. The increase of BDNF mRNA expression in 
      the cerebral cortex and hippocampus of experimental animals was demonstrated at 
      24 h after inoculation: (0.1194 +/- 0.02941 compared with 0.0662 +/- 0.01176)A 
      and (0.1608 +/-0.01854 compared with 0.0680 +/- 0.00946)A (P<0.01), respectively. 
      Compared with controls the expression of BDNF protein in the cerebral cortex and 
      hippocampus was enhanced at 24 h of inoculation:(177.04+/-43.66 compared with 
      79.79+/-7.23)mm(2) (P<0.01) and (81.78 +/-37.47 compared with 42.98 
      +/-20.44)mm(2) (P<0.01), respectively. Strong positive hybridization and 
      immunoreactivity were observed in the infiltrated inflammatory cell in 
      leptomeninges, subarachnoid cavity, ventricles and brain parenchyma in the brain 
      from the experimental rats. CONCLUSION: The expression of BDNF mRNA and BDNF 
      protein increases following brain inflammatory injury, which supports the 
      hypothesis that BDNF may constitute intrinsic neuroprotective mechanism as a part 
      of the inflammatory response.
FAU - Li, Ling
AU  - Li L
AD  - The Affiliated Children's Hospital, College of Medicine, Zhejiang University, 
      Hangzhou 310006, China.
FAU - Shui, Quan-xiang
AU  - Shui QX
FAU - Yu, Xi-lin
AU  - Yu XL
FAU - Shang, Shi-qiang
AU  - Shang SQ
FAU - Gu, Wei-zhong
AU  - Gu WZ
FAU - Tang, Hong-feng
AU  - Tang HF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhejiang Da Xue Xue Bao Yi Xue Ban
JT  - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical 
      sciences
JID - 100927946
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics
MH  - Calcium/metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - Male
MH  - Meningitis, Pneumococcal/*metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/11/12 05:00
MHDA- 2004/01/24 05:00
CRDT- 2003/11/12 05:00
PHST- 2003/11/12 05:00 [pubmed]
PHST- 2004/01/24 05:00 [medline]
PHST- 2003/11/12 05:00 [entrez]
AID - 10.3785/j.issn.1008-9292.2003.05.015 [doi]
PST - ppublish
SO  - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2003 Oct;32(5):433-6. doi: 
      10.3785/j.issn.1008-9292.2003.05.015.