PMID- 14611813
OWN - NLM
STAT- MEDLINE
DCOM- 20031224
LR  - 20190911
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 75
IP  - 3
DP  - 2003 Dec
TI  - Function of CD80 and CD86 on monocyte- and stem cell-derived dendritic cells.
PG  - 217-27
AB  - Dendritic cells (DCs) consist of a heterogeneous population of hematopoietic 
      cells characterized by their unique dendritic morphology, their efficient 
      antigen-presenting capability to activate naive CD4+ and CD8+ T cells, as well as 
      their lack of lineage-specific markers. Functional properties comparing umbilical 
      cord blood monocyte-derived and umbilical cord blood stem cell-derived DCs have 
      not yet been investigated. Human umbilical cord blood CD14+ monocytes and CD34+ 
      stem cells were induced to differentiate into dendritic cells using 100 ng/mL 
      granulocyte-macrophage colony-stimulating factor (GM-CSF), 25 ng/mL interleukin 
      (II)-4, 2.5 ng/mL tumor necrosis factor alpha (TNF-alpha) and 100 ng/mL GM-CSF, 
      25 ng/mL stem cell factor, and 2.5 ng/mL TNF-alpha, respectively. Differentiated 
      dendritic cells were CD80+, CD86+, CD83+, CD54+, CD1a+, CD11b+, CD11c+, HLA-DR+, 
      CD34-, CD3-, CD19-, CD14-, and CD16-. Reverse transcription polymerase chain 
      reaction revealed that differentiating monocytes initially expressed CD86 mRNA 
      while CD80 mRNA appeared on Day 2. Differentiating stem cells expressed both CD80 
      and CD86 mRNA on Day 2 of culture. Mixed lymphocyte reaction was employed to 
      evaluate the two types of lineage-derived DCs. Monoclonal antibodies (mabs) to 
      CD80 and CD86 were employed to assess their costimulatory roles. CD14 and CD34 
      derived DCs prior to the functional assay were stimulated for 18 h with 0.1 and 
      1.0 mg/mL Escherichia coli lipopolyssacharide, respectively. A decrease in 
      stimulation as depicted by decreased T-cell activation was significant with mabs 
      to both CD80 and CD86 on monocyte-derived DCs while only mabs to CD86 induced 
      decreased T-cell activation by stem cell-derived DCs. The varied functional role 
      of CD80 and CD86 costimulatory molecules is associated with DC differentiation 
      from distinct cord blood-isolated hematopoietic lineages. These studies 
      demonstrate that DC association with distinct hematopoietic lineages is of 
      relevance in transplantation and vaccine therapies.
FAU - Dilioglou, Smaroula
AU  - Dilioglou S
AD  - Department of Pathology, University of Mississippi Medical Center, Jackson, MS 
      39216-4505, USA. smaroula.dilioglou@stjude.org
FAU - Cruse, Julius M
AU  - Cruse JM
FAU - Lewis, Robert E
AU  - Lewis RE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD86 protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Antigens, CD/*physiology
MH  - B7-1 Antigen/*physiology
MH  - B7-2 Antigen
MH  - Cell Differentiation/*physiology
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology/metabolism
MH  - Fetal Blood/cytology
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/cytology/metabolism
MH  - Humans
MH  - Lymphocyte Culture Test, Mixed
MH  - Macrophage Activation/immunology
MH  - Membrane Glycoproteins/*physiology
MH  - Monocytes/cytology/metabolism
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology
EDAT- 2003/11/13 05:00
MHDA- 2003/12/25 05:00
CRDT- 2003/11/13 05:00
PHST- 2003/11/13 05:00 [pubmed]
PHST- 2003/12/25 05:00 [medline]
PHST- 2003/11/13 05:00 [entrez]
AID - S0014480003000728 [pii]
AID - 10.1016/s0014-4800(03)00072-8 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2003 Dec;75(3):217-27. doi: 10.1016/s0014-4800(03)00072-8.