PMID- 14611814
OWN - NLM
STAT- MEDLINE
DCOM- 20031224
LR  - 20190911
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 75
IP  - 3
DP  - 2003 Dec
TI  - Dendritic cell differentiation and proliferation: enhancement by tumor necrosis 
      factor-alpha.
PG  - 228-37
AB  - Dendritic cells (DCs) are a diverse group of hematopoietic-derived cells that 
      play a prominent role in initiating the body's immune response. Tumor necrosis 
      factor-alpha (TNFalpha) aids CD34+ hematopoietic stem cells in the development of 
      DCs. In this study, we aimed to further define the relationship between TNFalpha 
      and DC maturation. CD34+ stem cells were isolated from umbilical cord blood and 
      cultured using granulocyte-macrophage colony stimulating factor, stem cell 
      factor, and varying concentrations of TNFalpha. An anti-TNF receptor 1 
      (anti-TNFR1) antibody was used to show the specificity of TNFalpha. Flow 
      cytometry and light microscopy analyses were performed at days 0, 7, and 14 of 
      culture, revealing mature DCs at all concentrations of TNFalpha by day 14, 
      excluding those with anti-TNFR1 bound to the cell's TNF receptor 1. DCs possessed 
      a characteristic veiled appearance and were consistent with a DC panel of surface 
      markers. TNFalpha was essential to the development of DCs, as those with bound 
      anti-TNFR1 were virtually unable to develop into DCs. Increasing TNFalpha 
      enhanced the survival of culturing stem cells and resulted in a parallel increase 
      in day 14 DCs. Although increases in TNFalpha produced more DCs, these cells were 
      not as phenotypically mature, expressing less CD80 than those receiving only a 
      single initial dosage of TNFalpha. These studies support the prevalence of large 
      numbers of DCs under inflammatory conditions, such as the rheumatoid joint, where 
      local concentrations of TNFalpha are high.
FAU - Morrison, Robert S 3rd
AU  - Morrison RS 3rd
AD  - Department of Pathology, School of Medicine, University of Mississippi Medical 
      Center, 2500 North State Street, Jackson, MI 39216, USA.
FAU - Cruse, Julius M
AU  - Cruse JM
FAU - Wang, Huan
AU  - Wang H
FAU - Lewis, Robert E
AU  - Lewis RE
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Antigens, CD34)
RN  - 0 (B7-1 Antigen)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Antigens, CD34/metabolism
MH  - B7-1 Antigen/metabolism
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cell Division/drug effects/physiology
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Fetal Blood/cytology
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/cytology/drug effects
MH  - Humans
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2003/11/13 05:00
MHDA- 2003/12/25 05:00
CRDT- 2003/11/13 05:00
PHST- 2003/11/13 05:00 [pubmed]
PHST- 2003/12/25 05:00 [medline]
PHST- 2003/11/13 05:00 [entrez]
AID - S001448000300087X [pii]
AID - 10.1016/s0014-4800(03)00087-x [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2003 Dec;75(3):228-37. doi: 10.1016/s0014-4800(03)00087-x.