PMID- 14611909
OWN - NLM
STAT- MEDLINE
DCOM- 20040716
LR  - 20190827
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 90
IP  - 1
DP  - 2003 Nov 15
TI  - Serum levels of chemokines correlate with disease activity in patients with 
      retinal vasculitis.
PG  - 59-64
AB  - Retinal vasculitis (RV) is characterised pathologically by migration of 
      leucocytes across the blood-retinal barrier leading to oedema and photoreceptor 
      cell dysfunction. Chemokines are a family of small molecules involved in 
      leucocyte migration. In this study, levels of chemokines were measured in serum 
      from patients with RV and correlated with disease activity and drug treatment. 
      Serum samples (n= 100; 25 active, 75 inactive) were obtained from 50 patients 
      with RV, and levels of the chemokines MIP-1alpha, macrophage inflammatory 
      protein-1beta (MIP-1beta) and monocyte chemoattractant protein-1 (MCP-1) were 
      measured by ELISA. For longitudinal analysis levels of the same chemokines were 
      measured in six consecutive serum samples from 10 of the above patients. 
      Chemokine levels were correlated with disease activity and current drug treatment 
      for each sample. Sera from 20 healthy individuals were used as control samples. 
      Serum levels of MIP-1beta were significantly raised in patients with RV, whether 
      active or not, compared to healthy controls (P= 0.04). Levels of MIP-1beta and 
      MCP-1 correlated with disease activity in some patients and with prednisolone 
      levels in patients on this treatment alone. MIP-1alpha levels were not detectable 
      in all samples but were present in significantly more samples from patients with 
      active or inactive RV compared with healthy controls. Serum levels of the 
      chemokines MIP-1beta and MIP-1alpha, but not MCP-1 were raised in patients with 
      retinal vasculitis. Longitudinal analysis suggested that MIP-1beta and MCP-1 
      levels were controlled by drug treatment, particularly prednisolone. These data 
      demonstrate that chemokines are involved in the pathogenesis of RV and may act as 
      novel therapeutic targets.
FAU - Wallace, Graham R
AU  - Wallace GR
AD  - Department of Ophthalmology, GKT, St. Thomas' Hospital, London SE1 7EH, UK. 
      g.r.wallace@bham.ac.uk
FAU - Farmer, Ian
AU  - Farmer I
FAU - Church, Alison
AU  - Church A
FAU - Graham, Elizabeth M
AU  - Graham EM
FAU - Stanford, Miles R
AU  - Stanford MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 9PHQ9Y1OLM (Prednisolone)
SB  - IM
MH  - Chemokine CCL2/analysis/*blood/metabolism
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Macrophage Inflammatory Proteins/analysis/*blood/metabolism
MH  - Prednisolone/administration & dosage/therapeutic use
MH  - Recurrence
MH  - Retinal Vasculitis/*blood/pathology
EDAT- 2003/11/13 05:00
MHDA- 2004/07/17 05:00
CRDT- 2003/11/13 05:00
PHST- 2003/11/13 05:00 [pubmed]
PHST- 2004/07/17 05:00 [medline]
PHST- 2003/11/13 05:00 [entrez]
AID - S0165247803001597 [pii]
AID - 10.1016/s0165-2478(03)00159-7 [doi]
PST - ppublish
SO  - Immunol Lett. 2003 Nov 15;90(1):59-64. doi: 10.1016/s0165-2478(03)00159-7.