PMID- 14612923
OWN - NLM
STAT- MEDLINE
DCOM- 20040720
LR  - 20181130
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 23
IP  - 6
DP  - 2003 Dec
TI  - In situ detection of telomeres by fluorescence in situ hybridization and 
      telomerase activity in glioblastoma multiforme: correlation with p53 status, 
      EGFR, c-myc, MIB1, and Topoisomerase IIalpha protein expression.
PG  - 1529-35
AB  - Aberrations of genes/proteins regulating cell cycle and growth, increased 
      proliferation and telomerase activity (TA) are documentable in glioblastoma 
      multiforme. TA is more frequently detectable in secondary glioblastoma, which is 
      also characterized by p53 mutation/overexpression. Discordant telomere (Te) 
      length values have been reported in glioblastomas with and without TA. In 31 
      glioblastomas, in which pre-existing astrocytoma was not documented, we compared 
      cases with and without TA for the expression of p53, EGFR, c-Myc, MIB-1 and 
      Topoisomerase IIalpha; p53 mutations were also investigated by SSCP-PCR. 
      Correlations were made with Te parameters [TePs: number (TeNo), length and area] 
      as evaluated by image analysis in interphase nuclei of fluorescence in situ 
      hybridization (FISH)-processed sections. We found no differences in the 
      expression of the proteins evaluated and in TePs, except Te/nuclear area %, which 
      was significantly lower in TA+ cases (p=0.02). TePs were, instead, inversely 
      correlated with TA (p=0.0001). TA was positively correlated with MIB1 staining 
      index in the TA+ cases (p=0.033), which also showed a positive correlation 
      between TeNo and EGFR expression (p=0.042), and a trend towards a negative 
      correlation between TeNo and p53 expression (p=0.05). Tumors overexpressing EGFR 
      had a significantly shorter lifetime (p=0.0001). TeNo seems to be inversely 
      correlated to tumor proliferation and lifetime in glioblastoma multiforme.
FAU - Miracco, Clelia
AU  - Miracco C
AD  - Dipartimento di Patologia Umana e Oncologia, Sezione di Anatomia Patologica, 
      Policlinico Le Scotte, I-53100 Siena, Italy. miracco@unisi.it
FAU - De Santi, M Margherita
AU  - De Santi MM
FAU - Luzi, Pietro
AU  - Luzi P
FAU - Lalinga, Anna Vittoria
AU  - Lalinga AV
FAU - Laurini, Lorella
AU  - Laurini L
FAU - De Nisi, Maria Caterina
AU  - De Nisi MC
FAU - Angeloni, Giuseppina
AU  - Angeloni G
FAU - Brogi, Marco
AU  - Brogi M
FAU - Cardone, Concetta
AU  - Cardone C
FAU - Carducci, Antonietta
AU  - Carducci A
FAU - Arcuri, Felice
AU  - Arcuri F
FAU - Tosi, Piero
AU  - Tosi P
FAU - Rubino, Giovanni
AU  - Rubino G
FAU - Pirtoli, Luigi
AU  - Pirtoli L
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.7.49 (Telomerase)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
RN  - EC 5.99.1.3 (DNA Topoisomerases, Type II)
SB  - IM
MH  - Acid Phosphatase/metabolism
MH  - Adolescent
MH  - Adult
MH  - Antigens, Neoplasm
MH  - Brain Neoplasms/enzymology
MH  - Cell Division
MH  - Child
MH  - DNA Topoisomerases, Type II/metabolism
MH  - DNA-Binding Proteins
MH  - ErbB Receptors/metabolism
MH  - Glioblastoma/*enzymology/metabolism/pathology
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Isoenzymes/metabolism
MH  - Ki-67 Antigen/biosynthesis/metabolism
MH  - Middle Aged
MH  - Polymorphism, Single-Stranded Conformational
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Telomerase/*metabolism
MH  - Telomere/*ultrastructure
MH  - Tumor Suppressor Protein p53/metabolism
EDAT- 2003/11/13 05:00
MHDA- 2004/07/21 05:00
CRDT- 2003/11/13 05:00
PHST- 2003/11/13 05:00 [pubmed]
PHST- 2004/07/21 05:00 [medline]
PHST- 2003/11/13 05:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2003 Dec;23(6):1529-35.