PMID- 14613207
OWN - NLM
STAT- MEDLINE
DCOM- 20040712
LR  - 20031112
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 24
IP  - 21
DP  - 2003 Nov
TI  - Use of high-molecular-mass polyacrylamides as matrices for microchip 
      electrophoresis of DNA fragments.
PG  - 3793-9
AB  - DNA fragment analysis requires the use of polymer solutions as sieving matrices. 
      Generally, such matrices are constituted of high-molar-weight polymers employed 
      at a concentration higher than their entanglement threshold concentration. These 
      polymer solutions are highly viscous and difficult to use in the narrow channels 
      of a microchip. Ultralarge polyacrylamides synthesized via a nonconventional 
      method, being the low-temperature plasma-induced polymerization (PIP), were used 
      as DNA sieving matrices for microchip electrophoresis. The distinctive features 
      of these polymers (ultralarge molecular mass and linearity) allow their use at a 
      dilute concentration. Dilute PIP polyacrylamides revealed a constant value of 
      resolution in a broad range of DNA fragment sizes (123 bp-1353 bp), thus proving 
      to be effective in common genotyping applications. Moreover, the low viscosity of 
      the dilute solutions enable it to be easier and faster in filling the channel 
      between runs, thus enhancing the throughput of the microchip devices.
FAU - Cretich, Marina
AU  - Cretich M
AD  - Istituto di Chimica del Riconoscimento Molecolare, C.N.R., Milano, Italy. 
      marina.cretich@icrm.cnr.it
FAU - Chiari, Marcella
AU  - Chiari M
FAU - Rech, Ivan
AU  - Rech I
FAU - Cova, Sergio
AU  - Cova S
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Acrylic Resins)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Polydeoxyribonucleotides)
RN  - 0 (polyacrylamide gels)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Acrylic Resins
MH  - Base Pairing
MH  - DNA/*chemistry
MH  - Electrophoresis, Polyacrylamide Gel/instrumentation/*methods
MH  - Equipment Design
MH  - Indicators and Reagents
MH  - Miniaturization/instrumentation/methods
MH  - Oligodeoxyribonucleotides/*chemistry/isolation & purification
MH  - Oligonucleotide Array Sequence Analysis/instrumentation/*methods
MH  - Polydeoxyribonucleotides/*chemistry/isolation & purification
EDAT- 2003/11/13 05:00
MHDA- 2004/07/13 05:00
CRDT- 2003/11/13 05:00
PHST- 2003/11/13 05:00 [pubmed]
PHST- 2004/07/13 05:00 [medline]
PHST- 2003/11/13 05:00 [entrez]
AID - 10.1002/elps.200305595 [doi]
PST - ppublish
SO  - Electrophoresis. 2003 Nov;24(21):3793-9. doi: 10.1002/elps.200305595.