PMID- 14614267
OWN - NLM
STAT- MEDLINE
DCOM- 20031204
LR  - 20130131
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 3
IP  - 1
DP  - 2002 Mar
TI  - Expression of open reading frame 5 protein of porcine reproductive and 
      respiratory syndrome virus using semliki forest virus expression system.
PG  - 13-8
AB  - The ORF5 gene encodes a major envelope glycoprotein (GP5), which is one of the 
      three major proteins of porcine reproductive and respiratory syndrome virus 
      (PRRSV). The GP5 protein has been known to be a 24.5-26 kDa N-glycosylated 
      envelope protein. The GP5 is involved in inducing neutralizing antibodies. For 
      this reason, the GP5 is primary candidate for the PRRSV subunit vaccine. To 
      produce the native form of GP5 in mammalian cells, we have cloned the ORF5 gene 
      from PRRSV CNV-1 into the Semliki Forest virus (SFV)-based expression vector, 
      resulting in recombinant pSFV-ORF5. By the infection with recombinant pSFV-ORF5 
      to BHK-21 cells, the GP5 expression was confirmed by immunocytochemistry and 
      immunoblotting assay. The recombinant virus particle harboring ORF5 gene was 
      infectious to BHK-21 and MARC-145. The RNA synthesis and expression of GP5 in the 
      infected cell was also confirmed by RT-PCR.
FAU - Jung, Hae-Sun
AU  - Jung HS
AD  - College of Veterinary Medicine, Chungnam National University, Daejeon, 305-764, 
      Korea.
FAU - Hwang, In-Wook
AU  - Hwang IW
FAU - Kim, Su-Mi
AU  - Kim SM
FAU - Kim, Chul-Joong
AU  - Kim CJ
FAU - Shin, Kwang-Soon
AU  - Shin KS
FAU - Kim, Hyun-Soo
AU  - Kim HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (DNA Primers)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - *Genes, Viral
MH  - Plasmids/genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Restriction Mapping
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Semliki forest virus/*genetics
MH  - Swine
MH  - Viral Envelope Proteins/genetics
MH  - Viral Proteins/*genetics
MH  - Virology/methods
EDAT- 2003/11/14 05:00
MHDA- 2003/12/05 05:00
CRDT- 2003/11/14 05:00
PHST- 2003/11/14 05:00 [pubmed]
PHST- 2003/12/05 05:00 [medline]
PHST- 2003/11/14 05:00 [entrez]
AID - 200203013 [pii]
PST - ppublish
SO  - J Vet Sci. 2002 Mar;3(1):13-8.