PMID- 14614784
OWN - NLM
STAT- MEDLINE
DCOM- 20040707
LR  - 20181130
IS  - 1471-2202 (Electronic)
IS  - 1471-2202 (Linking)
VI  - 4
DP  - 2003 Nov 13
TI  - Human neuroepithelial cells express NMDA receptors.
PG  - 28
AB  - L-glutamate, an excitatory neurotransmitter, binds to both ionotropic and 
      metabotropic glutamate receptors. In certain parts of the brain the BBB contains 
      two normally impermeable barriers: 1) cerebral endothelial barrier and 2) 
      cerebral epithelial barrier. Human cerebral endothelial cells express NMDA 
      receptors; however, to date, human cerebral epithelial cells (neuroepithelial 
      cells) have not been shown to express NMDA receptor message or protein. In this 
      study, human hypothalamic sections were examined for NMDA receptors (NMDAR) 
      expression via immunohistochemistry and murine neuroepithelial cell line (V1) 
      were examined for NMDAR via RT-PCR and Western analysis. We found that human 
      cerebral epithelium express protein and cultured mouse neuroepithelial cells 
      express both mRNA and protein for the NMDA receptor. These findings may have 
      important consequences for neuroepithelial responses during excitotoxicity and in 
      disease.
FAU - Sharp, Christopher D
AU  - Sharp CD
AD  - Department of Molecular and Cellular Physiology, LSU Health Sciences Center, 
      Shreveport, LA, USA. csharp@lsuhsc.edu
FAU - Fowler, M
AU  - Fowler M
FAU - Jackson, T H 4th
AU  - Jackson TH 4th
FAU - Houghton, J
AU  - Houghton J
FAU - Warren, A
AU  - Warren A
FAU - Nanda, A
AU  - Nanda A
FAU - Chandler, I
AU  - Chandler I
FAU - Cappell, B
AU  - Cappell B
FAU - Long, A
AU  - Long A
FAU - Minagar, A
AU  - Minagar A
FAU - Alexander, J S
AU  - Alexander JS
LA  - eng
PT  - Journal Article
DEP - 20031113
PL  - England
TA  - BMC Neurosci
JT  - BMC neuroscience
JID - 100966986
RN  - 0 (NMDA receptor A1)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - VH92ICR8HX (N-methyl D-aspartate receptor subtype 2A)
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/metabolism
MH  - Blotting, Western
MH  - Brain/blood supply/cytology/*metabolism
MH  - Cells, Cultured
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epithelial Cells/cytology/*metabolism
MH  - Humans
MH  - Hypothalamus/blood supply/cytology/metabolism
MH  - Immunohistochemistry
MH  - Mice
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, N-Methyl-D-Aspartate/*biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC293342
EDAT- 2003/11/15 05:00
MHDA- 2004/07/09 05:00
PMCR- 2003/11/13
CRDT- 2003/11/15 05:00
PHST- 2003/03/14 00:00 [received]
PHST- 2003/11/13 00:00 [accepted]
PHST- 2003/11/15 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2003/11/15 05:00 [entrez]
PHST- 2003/11/13 00:00 [pmc-release]
AID - 1471-2202-4-28 [pii]
AID - 10.1186/1471-2202-4-28 [doi]
PST - epublish
SO  - BMC Neurosci. 2003 Nov 13;4:28. doi: 10.1186/1471-2202-4-28.