PMID- 14614904
OWN - NLM
STAT- MEDLINE
DCOM- 20040301
LR  - 20190712
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 122
IP  - 2
DP  - 2003
TI  - Investigating the synergistic effect of combined neurotrophic factor 
      concentration gradients to guide axonal growth.
PG  - 381-9
AB  - Neurotrophic factors direct axonal growth toward the target tissue by a 
      concentration gradient, which is mediated through different tyrosine kinase cell 
      surface receptors. In this study, well-defined concentration gradients of 
      neurotrophic factors (NFs) allowed us to study the synergistic effect of 
      different NFs (e.g. nerve growth factor [NGF], neurotrophin-3 [NT-3] and 
      brain-derived neurotrophic factor [BDNF]) for axonal guidance of embryonic lumbar 
      dorsal root ganglion cells (DRGs). Effective guidance of DRG axons was achieved 
      with a minimum NGF concentration gradient of 133 ng/ml/mm alone, or combined NGF 
      and NT-3 concentration gradients of 80 ng/ml/mm each. Interestingly, the combined 
      concentration gradients of NGF and BDNF did not show any significant synergism at 
      the concentration gradients studied. The synergism observed between NGF and NT-3 
      indicates that axons may be guided over a 12.5 mm distance, which is 
      significantly greater than that of 7.5 mm calculated by us for NGF alone or that 
      of 2 mm observed by others.
FAU - Cao, X
AU  - Cao X
AD  - Department of Chemical Engineering and Applied Chemistry, University of Toronto, 
      200 College Street, Toronto, Ontario, Canada M5S 3E5.
FAU - Shoichet, M S
AU  - Shoichet MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Drug Combinations)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Axons/*drug effects/physiology
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Drug Synergism
MH  - Ganglia, Spinal/drug effects/physiology
MH  - Nerve Growth Factors/*pharmacology
EDAT- 2003/11/15 05:00
MHDA- 2004/03/03 05:00
CRDT- 2003/11/15 05:00
PHST- 2003/11/15 05:00 [pubmed]
PHST- 2004/03/03 05:00 [medline]
PHST- 2003/11/15 05:00 [entrez]
AID - S0306452203006523 [pii]
AID - 10.1016/j.neuroscience.2003.08.018 [doi]
PST - ppublish
SO  - Neuroscience. 2003;122(2):381-9. doi: 10.1016/j.neuroscience.2003.08.018.