PMID- 14615882
OWN - NLM
STAT- MEDLINE
DCOM- 20040617
LR  - 20181130
IS  - 0028-1298 (Print)
IS  - 0028-1298 (Linking)
VI  - 368
IP  - 6
DP  - 2003 Dec
TI  - In vitro anti-inflammatory and pro-aggregative effects of a lipid compound, 
      petrocortyne A, from marine sponges.
PG  - 448-56
AB  - (3 S,14 S)-Petrocortyne A, a lipid compound (a C(46) polyacetylenic alcohol), 
      from marine sponges ( Petrosia sp.) is potently cytotoxic against several solid 
      tumour cells. In this study, we investigated in vitro anti-inflammatory and 
      pro-aggregative effects of petrocortyne A at non-cytotoxic concentrations on 
      various cellular inflammatory phenomena using the macrophage and monocytic cell 
      lines RAW264.7 and U937. Petrocortyne A blocked tumour necrosis factor-alpha 
      (TNF-alpha) production strongly and concentration-dependently in 
      lipopolysaccharide (LPS)-activated RAW264.7 cells and phorbol 12-myristate 
      13-acetate (PMA)/LPS-treated U937 cells. It also blocked NO production 
      concentration-dependently in LPS- or interferon (IFN)-gamma-treated RAW264.7 
      cells. Among the migration factors tested, the compound selectively blocked the 
      expression of hepatocyte growth factor/scatter factor (HGF/SF). On the other 
      hand, as assessed by a cell-cell adhesion assay, petrocortyne A did not block the 
      activation of adhesion molecules induced by aggregative antibodies to adhesion 
      molecules, but suppressed PMA-induced cell-cell adhesion significantly. 
      Intriguingly, petrocortyne A induced U937 homotypic aggregation following long 
      exposure (2 and 3 days), accompanied by weak induction of pro-aggregative signals 
      such as tyrosine phosphorylation of p132 and phosphorylation of extracellular 
      signal-related kinase 1 and 2 (ERK 1/2). Petrocortyne A may thus inhibit cellular 
      inflammatory processes and immune cell migration to inflamed tissue.
FAU - Hong, Sungyoul
AU  - Hong S
AD  - Department of Genetic Engineering, Sungkyunkwan University, 440-746, Suwon, South 
      Korea.
FAU - Kim, Sung Hwan
AU  - Kim SH
FAU - Rhee, Man Hee
AU  - Rhee MH
FAU - Kim, Ae Ra
AU  - Kim AR
FAU - Jung, Jee H
AU  - Jung JH
FAU - Chun, Taehoon
AU  - Chun T
FAU - Yoo, Eun Sook
AU  - Yoo ES
FAU - Cho, Jae Youl
AU  - Cho JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031113
PL  - Germany
TA  - Naunyn Schmiedebergs Arch Pharmacol
JT  - Naunyn-Schmiedeberg's archives of pharmacology
JID - 0326264
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Fatty Alcohols)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Polymers)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 201990-21-8 (petrocortyne A)
RN  - 25067-58-7 (Polyynes)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - 82115-62-6 (Interferon-gamma)
RN  - DH2M523P0H (Genistein)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - H88EPA0A3N (Staurosporine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - OC7TV75O83 (Acetylene)
SB  - IM
MH  - Acetylene/*analogs & derivatives/chemistry/*pharmacology
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Autocrine Communication/drug effects/physiology
MH  - Cell Adhesion Molecules/biosynthesis
MH  - Cell Aggregation/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Fatty Alcohols/*pharmacology
MH  - Genistein/pharmacology
MH  - Hepatocyte Growth Factor/biosynthesis
MH  - Humans
MH  - Insulin-Like Growth Factor II/biosynthesis
MH  - Interferon-gamma/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/metabolism
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Nitric Oxide/biosynthesis
MH  - Phosphorylation
MH  - Polymers/chemistry/*pharmacology
MH  - Polyynes
MH  - Porifera/*chemistry
MH  - Recombinant Proteins
MH  - Staurosporine/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/biosynthesis
MH  - U937 Cells
EDAT- 2003/11/15 05:00
MHDA- 2004/06/18 05:00
CRDT- 2003/11/15 05:00
PHST- 2003/08/18 00:00 [received]
PHST- 2003/10/18 00:00 [accepted]
PHST- 2003/11/15 05:00 [pubmed]
PHST- 2004/06/18 05:00 [medline]
PHST- 2003/11/15 05:00 [entrez]
AID - 10.1007/s00210-003-0848-7 [doi]
PST - ppublish
SO  - Naunyn Schmiedebergs Arch Pharmacol. 2003 Dec;368(6):448-56. doi: 
      10.1007/s00210-003-0848-7. Epub 2003 Nov 13.