PMID- 14616104
OWN - NLM
STAT- MEDLINE
DCOM- 20040414
LR  - 20220321
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 58
IP  - 12
DP  - 2003 Dec
TI  - Serological characteristics of peanut allergy in children.
PG  - 1285-92
AB  - BACKGROUND: Food challenge is considered an excellent clinical tool for the 
      diagnosis of specific food allergy. However in the case of peanut allergy it may 
      be difficult to perform because of the severity of the reactions. The 
      quantitation of a specific immunoglobulin E (IgE) response to different peanut 
      allergens could also contribute to the improvement of the diagnosis. We 
      characterized the IgE response to a whole peanut protein extract and to Ara h 1 
      and Ara h 2 in different groups of patients classified according to the severity 
      of their allergic reactions. METHODS: Specific serum IgE were analyzed in 96 
      children by enzyme-linked immunosorbent assay tests using a whole protein extract 
      or purified peanut proteins and anti-human IgE monoclonal antibodies labeled with 
      acetylcholinesterase. RESULTS: A parallel was observed between levels of 
      peanut-specific IgE and the classification in five groups and subgroups of 
      patients upon increasing severity of symptoms, especially within the group of 
      highest severity. Moreover, the highest frequency of positive response and the 
      highest levels of specific IgE were observed with whole peanut protein extract. 
      CONCLUSION: In a retrospective evaluation of peanut allergy in children, we have 
      shown that quantitation of peanut-specific IgE could be used to avoid a food 
      challenge particularly in the case of severe reactions. When compared to Ara h 1 
      and Ara h 2, whole peanut protein extract appeared to be the most appropriate 
      allergen to perform the test.
FAU - Bernard, H
AU  - Bernard H
AD  - INRA-Laboratoire d'Immuno-Allergie Alimentaire, CEA-Saclay, Gif sur Yvette, 
      France.
FAU - Paty, E
AU  - Paty E
FAU - Mondoulet, L
AU  - Mondoulet L
FAU - Burks, A W
AU  - Burks AW
FAU - Bannon, G A
AU  - Bannon GA
FAU - Wal, J M
AU  - Wal JM
FAU - Scheinmann, P
AU  - Scheinmann P
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (2S Albumins, Plant)
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Ara h 1 protein, Arachis hypogaea)
RN  - 0 (Ara h II protein, Arachis hypogaea)
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - 2S Albumins, Plant
MH  - Adolescent
MH  - Allergens/immunology
MH  - Antigens, Plant
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Glycoproteins/immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Infant
MH  - Male
MH  - Membrane Proteins
MH  - Peanut Hypersensitivity/diagnosis/*immunology
MH  - Plant Proteins/immunology
MH  - Retrospective Studies
MH  - Serologic Tests/methods
MH  - Severity of Illness Index
EDAT- 2003/11/18 05:00
MHDA- 2004/04/15 05:00
CRDT- 2003/11/18 05:00
PHST- 2003/11/18 05:00 [pubmed]
PHST- 2004/04/15 05:00 [medline]
PHST- 2003/11/18 05:00 [entrez]
AID - 300 [pii]
AID - 10.1046/j.1398-9995.2003.00300.x [doi]
PST - ppublish
SO  - Allergy. 2003 Dec;58(12):1285-92. doi: 10.1046/j.1398-9995.2003.00300.x.