PMID- 14616106
OWN - NLM
STAT- MEDLINE
DCOM- 20040414
LR  - 20190823
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 58
IP  - 12
DP  - 2003 Dec
TI  - Characterization of allergens of Anisakis simplex.
PG  - 1299-303
AB  - BACKGROUND: Anisakis simplex is an intestinal parasite of sea mammals. The larvae 
      infect crustaceans, cephalopods and fish. Humans may consume A. simplex third 
      stage larvae (L3) when eating infected raw or under-cooked fish. Consumed larvae 
      cause an inflammatory reaction when they penetrate the digestive mucosa. The 
      larvae or their secretory/excretory products can sensitize humans and induce an 
      immunoglobulin E (IgE)-mediated allergic reaction. This parasite is now being 
      implicated in numerous cases of allergic reactions after eating fish. The purpose 
      of this study was to evaluate the allergenicity of proteins present in an extract 
      of the third stage larva. METHODS: Rabbit antiserum raised to A. simplex somatic 
      extract (L3) was reacted by crossed immunoelectrophoresis (CIE) with the same 
      somatic extract. Crossed radioimmunoelectrophoresis (CRIE) was also performed by 
      incubating CIE gels first in the sera of 13 individuals with positive immunoCAP 
      to A. simplex and then in radiolabeled anti-human IgE. RESULTS: Twelve to 16 
      antigen-antibody precipitin peaks were visualized on Coomassie blue stained CIE 
      gels in which somatic extract was reacted with somatic-antiserum. Autoradiography 
      of CRIE gels showed that 18 different proteins bound IgE in patient sera. 
      Individual patients had serum IgE directed at two to 10 different allergens. Five 
      of these allergens were recognized by >/=50% of the patients. No allergen was 
      recognized by every patient and no patient had serum IgE directed at all 18 
      allergens. CONCLUSION: Somatic extracts of A. simplex L3 larva contain a large 
      number of allergenic molecules and there is significant variability between 
      patients in their sensitivity and reactivity to these allergens.
FAU - Arlian, L G
AU  - Arlian LG
AD  - Department of Biological Sciences, Wright State University, Dayton, OH 45435, 
      USA.
FAU - Morgan, M S
AU  - Morgan MS
FAU - Quirce, S
AU  - Quirce S
FAU - Maranon, F
AU  - Maranon F
FAU - Fernandez-Caldas, E
AU  - Fernandez-Caldas E
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Allergens/*immunology
MH  - Animals
MH  - Anisakis/*immunology
MH  - Humans
MH  - Immunoelectrophoresis/methods
MH  - Middle Aged
EDAT- 2003/11/18 05:00
MHDA- 2004/04/15 05:00
CRDT- 2003/11/18 05:00
PHST- 2003/11/18 05:00 [pubmed]
PHST- 2004/04/15 05:00 [medline]
PHST- 2003/11/18 05:00 [entrez]
AID - 344 [pii]
AID - 10.1046/j.1398-9995.2003.00344.x [doi]
PST - ppublish
SO  - Allergy. 2003 Dec;58(12):1299-303. doi: 10.1046/j.1398-9995.2003.00344.x.