PMID- 14616127
OWN - NLM
STAT- MEDLINE
DCOM- 20040409
LR  - 20190823
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 58
IP  - 11
DP  - 2003 Nov
TI  - Characterization of a novel Candida albicans 29 kDa IgE-binding 
      protein--purification, cDNA isolation and heterologous expression of Cand a 3.
PG  - 1157-64
AB  - BACKGROUND: Candida albicans has been implicated in human allergic disorders. 
      However, many of its immunoglobulin E (IgE)-reacting components have not yet been 
      identified. The purpose of the present study is to characterize a novel 29 kDa 
      IgE-binding protein from C. albicans. METHODS: The 29 kDa protein was partially 
      purified and its tryptic digests subjected to mass spectrometric analysis. The 
      cDNA encoding this protein was isolated and heterologously expressed in 
      Escherichia coli. Monoclonal antibodies (MoAbs) were raised against the 29 kDa 
      protein purified from C. abicans extracts. RESULTS: We isolated a 29 kDa 
      IgE-reacting component from C. albicans. The protein was digested on-gel with 
      trypsin and the masses of the resulting fragments were determined in a MALDI-TOF 
      mass spectrometer. The data were searched against protein sequences deduced from 
      the C. albicans genome. An open reading frame that possibly encodes the 29 kDa 
      IgE-reacting component was identified. The cDNA corresponding to the open reading 
      frame was isolated. It encodes a 236 residues protein that has 62% sequence 
      identity to that of a hypothetical protein (YDR533c) from Saccharomyces 
      cerevisiae. Conserved domain search suggests that the encoded protein belongs to 
      the ThiJ/PfpI family. The cDNA isolated was inserted into a pQE-30 vector for 
      protein expression in Escherichia coli. The recombinant protein can react with 
      IgE antibodies in sera from asthmatic patients and two MoAbs that were generated 
      against the purified native 29 kDa protein from C. albicans. CONCLUSIONS: We 
      identified and cloned a novel 29 kDa IgE-reacting component (Cand a 3) from C. 
      albicans. The recombinant proteins produced from this clone and the MoAbs 
      prepared may be useful in the standardization of diagnostic extracts. They are 
      also instrumental in elucidating the role of C. albicans in clinical allergy.
FAU - Chou, H
AU  - Chou H
AD  - Department of Medical Research, Veterans General Hospital-Taipei, Taiwan.
FAU - Tam, M F
AU  - Tam MF
FAU - Chang, C-Y
AU  - Chang CY
FAU - Lai, H-Y
AU  - Lai HY
FAU - Huang, M-H
AU  - Huang MH
FAU - Chou, C-T
AU  - Chou CT
FAU - Lee, S-S
AU  - Lee SS
FAU - Shen, H-D
AU  - Shen HD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/immunology
MH  - Asthma/immunology
MH  - Base Sequence
MH  - Candida albicans/genetics/*immunology
MH  - DNA, Complementary/genetics
MH  - DNA, Fungal/genetics
MH  - Fungal Proteins/genetics/*immunology
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/*immunology
MH  - Middle Aged
MH  - Molecular Weight
MH  - Open Reading Frames
EDAT- 2003/11/18 05:00
MHDA- 2004/04/10 05:00
CRDT- 2003/11/18 05:00
PHST- 2003/11/18 05:00 [pubmed]
PHST- 2004/04/10 05:00 [medline]
PHST- 2003/11/18 05:00 [entrez]
AID - 275 [pii]
AID - 10.1034/j.1398-9995.2003.00275.x [doi]
PST - ppublish
SO  - Allergy. 2003 Nov;58(11):1157-64. doi: 10.1034/j.1398-9995.2003.00275.x.