PMID- 14616262
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20221207
IS  - 0001-6349 (Print)
IS  - 0001-6349 (Linking)
VI  - 82
IP  - 12
DP  - 2003 Dec
TI  - Alterations in the expression of selected MHC antigens in premalignant lesions 
      and squamous carcinomas of the uterine cervix.
PG  - 1146-52
AB  - BACKGROUND: The objective of the study was to determine whether there is a 
      correlation between expression of selected major histocompatibility complex (MHC) 
      antigens and the presence of carcinoma and premalignant lesions of the cervix. We 
      also attempted to determine whether there is a correlation between expression of 
      the selected MHC antigens in each grade of cervical intraepithelial neoplasia 
      (CIN) and invasive carcinoma of the cervix. METHODS: Quantitative comparative 
      analysis of MHC class I and class II expression between specimens of the uterine 
      cervix was carried out. For detecting human leukocyte antigen (HLA) class I 
      molecules, two primary antibodies were used, recognizing either epitopes of all 
      free heavy chain class I molecules or epitopes of the HLA-Bw4 antigen. HLA class 
      II antigens were detected by antibodies reacting with the alpha-chain of the 
      HLA-DR molecule. An indirect immunoperoxidase technique was carried out using 
      paraffin-embedded sections. The following groups of sections were formed: 
      squamous metaplasia, CIN of different grades, and invasive carcinoma. The 
      specimens were taken from women in whom routine histopathologic diagnosis of 
      cervical lesions had been performed. RESULTS: A significant decrease in the mean 
      number of stained cells showing expression of MHC class I antigens (HC 10 and 
      Bw4) was found in the invasive cancer group of specimens. A decrease in Bw4 
      expression was also found in the CIN III group. The highest expression of the 
      HLA-DR antigens was detected in the metaplastic epithelium compared to the other 
      groups. In the dysplastic epithelium the expression of the HLA-DR antigens was 
      increased in the highest grade of dysplasia (CIN III). Upregulation of HLA-DR 
      expression was detected in invasive cancer specimens. CONCLUSIONS: Alterations in 
      MHC expression are found in premalignant lesions and squamous carcinomas of the 
      uterine cervix. Quantitative assessment with the use of an image analysis system 
      is valuable in helping to discriminate staining patterns of HLA expression. 
      Further studies are indicated to evaluate the prognostic value of the expression 
      of selected MHC antigens.
FAU - Chil, Arkadiusz
AU  - Chil A
AD  - Department of Gynecologic Oncology, Kielce Oncology Center, Kielce, Poland. 
      archil@poczta.fm
FAU - Sikorski, Marek
AU  - Sikorski M
FAU - Bobek, Malgorzata
AU  - Bobek M
FAU - Jakiel, Grzegorz
AU  - Jakiel G
FAU - Marcinkiewicz, Janusz
AU  - Marcinkiewicz J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Acta Obstet Gynecol Scand
JT  - Acta obstetricia et gynecologica Scandinavica
JID - 0370343
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Biomarkers, Tumor/analysis
MH  - Biopsy, Needle
MH  - Carcinoma, Squamous Cell/*immunology/pathology
MH  - Cohort Studies
MH  - Diagnosis, Differential
MH  - Female
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Precancerous Conditions/*immunology/pathology
MH  - Prognosis
MH  - Sensitivity and Specificity
MH  - Uterine Cervical Neoplasms/immunology/*pathology
MH  - Uterine Cervical Dysplasia/*immunology/pathology
EDAT- 2003/11/18 05:00
MHDA- 2004/01/06 05:00
CRDT- 2003/11/18 05:00
PHST- 2003/11/18 05:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/11/18 05:00 [entrez]
AID - 252 [pii]
AID - 10.1046/j.1600-0412.2003.00252.x [doi]
PST - ppublish
SO  - Acta Obstet Gynecol Scand. 2003 Dec;82(12):1146-52. doi: 
      10.1046/j.1600-0412.2003.00252.x.