PMID- 14617837
OWN - NLM
STAT- MEDLINE
DCOM- 20040130
LR  - 20061115
IS  - 1234-1983 (Print)
IS  - 1234-1983 (Linking)
VI  - 44
IP  - 4
DP  - 2003
TI  - Molecular diagnostics of promyelocytic leukaemia.
PG  - 553-6
AB  - Acute promyelocytic leukaemia (APL) is characterised by proliferation of abnormal 
      promyelocytes. The reciprocal translocation between the long arms of chromosomes 
      15 and 17, and the fusion between the retinoic acid receptor (RARa) gene, and PML 
      gene, is unique to APL. Because of unsuccessful cytogenetic analysis of 
      conventional G-banding technique (mitoses were not observed), we diagnosed three 
      non-treatment patients with APL by following molecular methods: reverse 
      transcription-polymerase chain reaction (RT-PCR), fluorescence in situ 
      hybridization (FISH) and comparative genomic hybridization (CGH). At the time of 
      diagnosis our patients showed reciprocal translocation t(15;17)(q22;q12) in all 
      cases studied (66-85% of positive bone marrow cells). With the use of CGH we 
      observed the unbalanced chromosomal aberrations: losses of 5q13.1, 5q31.3, 9p21 
      regions, gain of 5q32 region and trisomy of 18 chromosome.
FAU - Kocki, Janusz
AU  - Kocki J
AD  - Department of Medical Genetics, Medical University of Lublin, Lublin, Poland. 
      janusz.kocki@inetia.pl
FAU - Constantinou, Maria
AU  - Constantinou M
FAU - Cioch, Maria
AU  - Cioch M
FAU - Lancut, Miroslaw
AU  - Lancut M
FAU - Kaluzewski, Bogdan
AU  - Kaluzewski B
FAU - Dmoszynska, Anna
AU  - Dmoszynska A
FAU - Wojcierowski, Jacek
AU  - Wojcierowski J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Promyelocytic, Acute/*diagnosis
MH  - Molecular Diagnostic Techniques
MH  - Nucleic Acid Hybridization
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/11/18 05:00
MHDA- 2004/01/31 05:00
CRDT- 2003/11/18 05:00
PHST- 2003/11/18 05:00 [pubmed]
PHST- 2004/01/31 05:00 [medline]
PHST- 2003/11/18 05:00 [entrez]
AID - 174 [pii]
PST - ppublish
SO  - J Appl Genet. 2003;44(4):553-6.