PMID- 14619573
OWN - NLM
STAT- MEDLINE
DCOM- 20040803
LR  - 20181217
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 34
IP  - 4
DP  - 2003 Oct
TI  - [Effects of adrenomedullin on secretory function of pancreatic islet in 
      spontaneously hypertensive rat in vitro].
PG  - 656-9
AB  - OBJECTIVE: To study the effects of adrenomedullin (AM) on secretory function of 
      pancreatic islet in spontaneously hypertensive rat (SHR) and Wistar-kyoto (WKY) 
      rat. METHODS: Ten SHRs (test group) and ten WKY rats (control group) were used. 
      The pancreatic islets isolated from SHR and WKY rats were put into 12-well 
      plates. The islets were incubated in RPMI-1640 medium containing 2.8 mmol/L 
      glucose (low glucose group) for 45 minutes. The second incubation was performed 
      in RPMI-1640 medium containing 11.1 mmol/L glucose (high glucose group) and 
      various concentrations of AM (0, 10(-8), 10(-7), 10(-6) mol/L, respectively) for 
      24 hours. The supernatants from both incubations were tested by RIA for insulin 
      and glucagon. RESULTS: The insulin concentrations in supernatants collected from 
      the islets of WKY rats incubated in high glucose and various concentrations of AM 
      for 24 hours were decreased in the dose dependent manner. When the islets of WKY 
      rats and SHR were incubated in AM of high concentration (10(-6) mol/L), the 
      insulin concentrations in supernatants decreased significantly. When the islets 
      of SHR were incubated in high glucose and various concentrations of AM for 24 
      hours, the glucagon concentrations in supernatants were not significantly 
      different. CONCLUSION: The above results demonstrate that AM inhibits insulin 
      secretion in beta cell of islets of WKY rats in dose dependent manner, that AM 
      that AM of high concentration inhibits insulin secretion in beta cell of islets 
      of SHR, and that AM has no influence on the function of alpha cell of islets. 
      These suggest that AM may contribute to the impaired beta cell function during 
      the development of diabetes mellitus.
FAU - Chen, Chuanqi
AU  - Chen C
AD  - Department of Endocrinology, West China Hospital, Sichuan University, Chengdu 
      610041, China.
FAU - Tong, Nanwei
AU  - Tong N
FAU - Zhang, Wenyi
AU  - Zhang W
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Insulin)
RN  - 0 (Peptides)
RN  - 148498-78-6 (Adrenomedullin)
RN  - 9007-92-5 (Glucagon)
SB  - IM
MH  - Adrenomedullin
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Glucagon/analysis/metabolism
MH  - Hypertension/*physiopathology
MH  - Insulin/analysis/*metabolism
MH  - Insulin Secretion
MH  - Islets of Langerhans/*metabolism
MH  - Male
MH  - Peptides/*pharmacology
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
EDAT- 2003/11/19 05:00
MHDA- 2004/08/04 05:00
CRDT- 2003/11/19 05:00
PHST- 2003/11/19 05:00 [pubmed]
PHST- 2004/08/04 05:00 [medline]
PHST- 2003/11/19 05:00 [entrez]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Oct;34(4):656-9.