PMID- 14620862
OWN - NLM
STAT- MEDLINE
DCOM- 20040126
LR  - 20181113
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 67
IP  - 4
DP  - 2003 Oct
TI  - Evaluation of mosquitoes, Aedes vexans, as biological vectors of porcine 
      reproductive and respiratory syndrome virus.
PG  - 265-70
AB  - The objective of this study was to determine whether mosquitoes, Aedes vexans 
      (Meigen), could serve as biological vectors of porcine reproductive and 
      respiratory syndrome virus (PRRSV). Specifically, the study assessed the duration 
      of viability and the site of PRRSV within mosquitoes, and evaluated whether PRRSV 
      could be transmitted to a susceptible pig by mosquitoes following a 7- to 14-day 
      incubation period after feeding on an infected pig. For the first experiment, a 
      total of 100 mosquitoes were allowed to feed on a pig, experimentally infected 
      with PRRSV (day 7 post-inoculation) and were then maintained alive under 
      laboratory conditions. A set of 10 mosquitoes were collected at 0 hour (h), 6 h, 
      12 h, 24 h, 48 h, 72 h, 5 days (d), 7 d, 10 d, and 14 d post-feeding (pf). 
      Samples of exterior surface washes, salivary glands, thorax carcasses, and gut 
      homogenates were collected from each set of mosquitoes, and tested for PRRSV. 
      Infectious PRRSV was detected by polymerase chain reaction and swine bioassay 
      only from the gut homogenates of mosquitoes collected at 0 h and 6 h pf. For the 
      second experiment, a total of 30 mosquitoes were allowed to feed on a pig, 
      experimentally infected with PRRSV and the mosquitoes were then maintained under 
      laboratory conditions. On each of day 7, 10, and 14 pf, a set of 10 mosquitoes 
      were allowed to feed on a susceptible pig. Transmission of PRRSV to susceptible 
      pigs did not occur, and PRRSV was not detected from the mosquitoes. These 
      findings indicate that mosquitoes are not likely to serve as biological vectors 
      of PRRSV.
FAU - Otake, Satoshi
AU  - Otake S
AD  - Swine Disease Eradication Center, University of Minnesota College of Veterinary 
      Medicine, St. Paul, Minnesota 55108, USA. otak0001@tc.umn.edu
FAU - Dee, Scott A
AU  - Dee SA
FAU - Moon, Roger D
AU  - Moon RD
FAU - Rossow, Kurt D
AU  - Rossow KD
FAU - Trincado, Carlos
AU  - Trincado C
FAU - Pijoan, Carlos
AU  - Pijoan C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Aedes/*virology
MH  - Animals
MH  - Biological Assay/veterinary
MH  - DNA, Viral/analysis
MH  - Insect Vectors/*virology
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*transmission/virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Swine
MH  - Time Factors
PMC - PMC280710
EDAT- 2003/11/19 05:00
MHDA- 2004/01/27 05:00
PMCR- 2004/01/01
CRDT- 2003/11/19 05:00
PHST- 2003/11/19 05:00 [pubmed]
PHST- 2004/01/27 05:00 [medline]
PHST- 2003/11/19 05:00 [entrez]
PHST- 2004/01/01 00:00 [pmc-release]
AID - 0000000-200310000-00003 [pii]
PST - ppublish
SO  - Can J Vet Res. 2003 Oct;67(4):265-70.