PMID- 14620872
OWN - NLM
STAT- MEDLINE
DCOM- 20040121
LR  - 20061115
IS  - 1050-9631 (Print)
IS  - 1050-9631 (Linking)
VI  - 13
IP  - 7
DP  - 2003
TI  - Differential expression and localization of the phosphorylated and 
      nonphosphorylated neurofilaments during the early postnatal development of rat 
      hippocampus.
PG  - 767-79
AB  - Neurofilament (NF) proteins are expressed in most mature neurons in the central 
      nervous system. Although they play a crucial role in neuronal growth, 
      organization, shape, and plasticity, their expression pattern and cellular 
      distribution in the developing hippocampus remain unknown. In the present study, 
      we have used Western blotting and immunocytochemistry to study the low- (NF-L), 
      medium- (NF-M), and high- (NF-H) molecular-weight NF proteins; phosphorylated 
      epitopes of NF-M and NF-H; and a nonphosphorylated epitope of NF-H in the early 
      postnatal (through P1-P21) development of the rat hippocampus. During the first 
      postnatal week, NF-M was the most abundantly expressed NF, followed by NF-L, 
      whereas the expression of NF-H was very low. Through P7-P14, the expression of 
      NF-H increased dramatically and later began to plateau, as also occurred in the 
      expression of NF-M and NF-L. At P1, no NF-M immunopositive cell bodies were 
      detected, but cell processes in the CA1-CA3 fields were faintly immunopositive 
      for NF-M and for the phosphorylated epitopes of NF-M and NF-H. At P7, CA3 
      pyramidal neurons were strongly immunopositive for NF-L and NF-H, but not for 
      NF-M. The axons of granule cells, the mossy fibers (MFs), were NF-L and NF-M 
      positive through P7-P21 but were NF-H immunonegative at all ages. Although they 
      stained strongly for the phosphorylated NF-M and NF-H at P7, the staining 
      intensity sharply decreased at P14 and remained so at P21. The cell bodies of CA1 
      pyramidal neurons and granule cells remained immunonegative against all five 
      antibodies in all age groups. Our results show a different time course in the 
      expression and differential cell type and cellular localization of the NF 
      proteins in the developing hippocampus. These developmental changes could be of 
      importance in determining the reactivity of hippocampal neurons in pathological 
      conditions in the immature hippocampus.
FAU - Lopez-Picon, F R
AU  - Lopez-Picon FR
AD  - Department of Pharmacology and Clinical Pharmacology, University of Turku, Turku, 
      Finland.
FAU - Uusi-Oukari, M
AU  - Uusi-Oukari M
FAU - Holopainen, I E
AU  - Holopainen IE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hippocampus
JT  - Hippocampus
JID - 9108167
RN  - 0 (Epitopes)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (neurofilament protein L)
RN  - 108688-71-7 (neurofilament protein H)
RN  - 111365-29-8 (neurofilament protein M)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation/*physiology
MH  - Dentate Gyrus/cytology/growth & development/metabolism
MH  - Epitopes/immunology/metabolism
MH  - Hippocampus/cytology/*growth & development/*metabolism
MH  - Immunohistochemistry
MH  - Mossy Fibers, Hippocampal/metabolism/ultrastructure
MH  - Neurofilament Proteins/immunology/*metabolism
MH  - Neurons/cytology/*metabolism
MH  - Phosphorylation
MH  - Pyramidal Cells/cytology/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2003/11/19 05:00
MHDA- 2004/01/22 05:00
CRDT- 2003/11/19 05:00
PHST- 2003/11/19 05:00 [pubmed]
PHST- 2004/01/22 05:00 [medline]
PHST- 2003/11/19 05:00 [entrez]
AID - 10.1002/hipo.10122 [doi]
PST - ppublish
SO  - Hippocampus. 2003;13(7):767-79. doi: 10.1002/hipo.10122.