PMID- 14620921
OWN - NLM
STAT- MEDLINE
DCOM- 20040624
LR  - 20191026
IS  - 0916-9636 (Print)
IS  - 0916-9636 (Linking)
VI  - 26
IP  - 9
DP  - 2003 Sep
TI  - Polymorphism of the monocyte chemoattractant protein (MCP-1) gene is associated 
      with the plasma level of MCP-1 but not with carotid intima-media thickness.
PG  - 677-83
AB  - Monocyte chemoattractant protein-1 (MCP-1) plays an important role in 
      atherosclerosis. Recently, single nucleotide polymorphisms (SNPs) in the MCP-1 
      regulatory region have been identified, and an in vitro study demonstrated that 
      the SNP at position -2518 of the MCP-1 gene affected transcription of the gene. 
      The purpose of this study was to clarify the association of the plasma level of 
      MCP-1 and the SNP of the MCP-1 gene with carotid atherosclerosis in 
      community-based subjects. The study subjects consisted of 325 community 
      residents, aged 50 years or older (mean age, 70.5 +/- 9.4 years) and free from 
      any cardiovascular complications. Carotid intima-media thickness (IMT) was 
      measured in the right common carotid artery using ultrasonography. The plasma 
      level of MCP-1 was measured by enzyme-linked immunosorbent assay (ELISA). The SNP 
      of the MCP-1 gene was determined by the polymerase chain reaction 
      (PCR)-restriction fragment length polymorphism (RFLP) technique. The plasma level 
      of MCP-1 was significantly associated with IMT (r = 0.12, p < 0.05) and carotid 
      arterial dimension (r = 0.13, p < 0.05). There was a significant difference in 
      plasma MCP-1 level between the genotypes (AA, 166 +/- 36 ng/ml; GG + AG, 184 +/- 
      56 ng/ml; p = 0.036). Analysis restricted to the subjects not receiving 
      antihypertensive drugs or other medication further increased the statistical 
      significance. However, carotid IMT and carotid arterial diameter were not 
      significantly different among the MCP-1 genotypes. Stepwise regression analysis 
      for plasma MCP-1 revealed that the MCP-1 genotype was an independent determinant 
      of plasma MCP-1 level. These findings indicate that plasma MCP-1 is associated 
      with carotid atherosclerosis. Although -2518 SNP is associated with the plasma 
      level of MCP-1, it was not directly associated with carotid atherosclerosis.
FAU - Tabara, Yasuharu
AU  - Tabara Y
AD  - Department of Medical Genetics, Ehime University School of Medicine, Ehime, 
      Japan.
FAU - Kohara, Katsuhiko
AU  - Kohara K
FAU - Yamamoto, Yoshikuni
AU  - Yamamoto Y
FAU - Igase, Michiya
AU  - Igase M
FAU - Nakura, Jun
AU  - Nakura J
FAU - Kondo, Ikuko
AU  - Kondo I
FAU - Miki, Tetsuro
AU  - Miki T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hypertens Res
JT  - Hypertension research : official journal of the Japanese Society of Hypertension
JID - 9307690
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Aged
MH  - Carotid Artery Diseases/diagnostic imaging/*genetics
MH  - Carotid Artery, Common/diagnostic imaging/metabolism/*pathology
MH  - Chemokine CCL2/*blood/*genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - Tunica Intima/diagnostic imaging/pathology
MH  - Tunica Media/diagnostic imaging/pathology
MH  - Ultrasonography
EDAT- 2003/11/19 05:00
MHDA- 2004/06/25 05:00
CRDT- 2003/11/19 05:00
PHST- 2003/11/19 05:00 [pubmed]
PHST- 2004/06/25 05:00 [medline]
PHST- 2003/11/19 05:00 [entrez]
AID - 10.1291/hypres.26.677 [doi]
PST - ppublish
SO  - Hypertens Res. 2003 Sep;26(9):677-83. doi: 10.1291/hypres.26.677.