PMID- 14621780
OWN - NLM
STAT- MEDLINE
DCOM- 20031223
LR  - 20190917
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 5 Suppl 1
DP  - 1992
TI  - Hepatic support by hepatocyte transplantation in congenitally metabolic diseased 
      rats.
PG  - S211-3
AB  - Nagase analbuminemic rats (NAR), which lack albumin synthesis in the liver, 
      underwent intrasplenic hepatocyte transplantation (HCTx), and the long-term 
      effects were studied using functional and morphological examinations. Hepatocytes 
      were isolated from congenic F344 rats with collagenase infusion, and 1 x 10(7) 
      cells were injected into the spleen of 3-month-old NAR (n = 10). Serum albumin 
      increased with time, reaching 53.9 mg/dl 14 months after HCTx, which was 
      equivalent to 2.1% (maximum 4%) of serum albumin in normal rats. On the other 
      hand, untreated NAR showed persistently low serum albumin levels (0.99 +/- 0.23 
      mg/dl at 10 months). According to immunostaining with anti-rat albumin antibody 
      at 16 months after HCTx, hepatocyte grafts occupied 27-41% of the spleen area and 
      weighed 120-420 mg, which was equivalent to 0.8-2.9% of a whole liver. Our study 
      demonstrated that grafted hepatocytes can grow in the spleen with the ability to 
      synthesize albumin. HCTx in NAR is a new experimental system to monitor the 
      function and survival of grafted heptocytes without sacrificing the animals by 
      measuring serum albumin levels. Certain manipulations to facilitate the growth of 
      grafted hepatocytes are necessary to achieve sufficient hepatic support in HCTx.
FAU - Nozawa, M
AU  - Nozawa M
AD  - Department of Surgery, Meikai University 1-1 Keyakidai, Sakado, Saitama, Japan.
FAU - Ikebukuro, H
AU  - Ikebukuro H
FAU - Otsu, I
AU  - Otsu I
FAU - Inagaki, M
AU  - Inagaki M
FAU - Ebata, H
AU  - Ebata H
FAU - Mito, M
AU  - Mito M
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Hepatocytes/*transplantation
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rats, Inbred Strains
MH  - Reference Values
MH  - Serum Albumin/analysis/*deficiency
MH  - Transplantation, Homologous
EDAT- 1992/01/01 00:00
MHDA- 2003/12/24 05:00
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 2003/12/24 05:00 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1007/978-3-642-77423-2_67 [doi]
PST - ppublish
SO  - Transpl Int. 1992;5 Suppl 1:S211-3. doi: 10.1007/978-3-642-77423-2_67.