PMID- 14622987
OWN - NLM
STAT- MEDLINE
DCOM- 20040213
LR  - 20191210
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 420
IP  - 1
DP  - 2003 Dec 1
TI  - Measurement of oxygen consumption in mouse aortic endothelial cells using a 
      microparticulate oximetry probe.
PG  - 169-75
AB  - The purpose of this study was to determine the rate of oxygen consumption in 
      mouse aortic endothelial cells (MAECs) and to determine the effect of a variety 
      of inhibitors and stimulators of oxygen consumption measured by electron 
      paramagnetic resonance (EPR) spectroscopy utilizing a new particulate oximetry 
      probe. We have previously demonstrated that the octa-n-butoxy derivative of 
      naphthalocyanine neutral radical (LiNc-BuO) enables accurate, precise, and 
      reproducible measurements of pO(2) in cellular suspensions. In the current study, 
      we carried out measurements to provide an accurate determination of pO(2) in 
      small volume with less number of cells (20,000 cells) that has not been possible 
      with other techniques. To establish the reliability of this method, agents such 
      as menadione, lipopolysaccharide (LPS), potassium cyanide, rotenone, and 
      diphenyleneiodonium chloride (DPI) were used to modulate the oxygen consumption 
      rate in the cells. We observed an increase in oxygen consumption by the cells 
      upon treatment with menadione and LPS, whereas treatment with cyanide, rotenone, 
      and DPI inhibited oxygen consumption. This study clearly demonstrated the 
      utilization of EPR spectrometry with LiNc-BuO probe for determination of oxygen 
      concentration in cultured cells.
FAU - Pandian, Ramasamy P
AU  - Pandian RP
AD  - Department of Internal Medicine, Center for Biomedical EPR Spectroscopy and 
      Imaging, Davis Heart and Lung Research Institute, The Ohio State University, 
      Columbus, OH 43210, USA.
FAU - Kutala, Vijay Kumar
AU  - Kutala VK
FAU - Parinandi, Narasimham L
AU  - Parinandi NL
FAU - Zweier, Jay L
AU  - Zweier JL
FAU - Kuppusamy, Periannan
AU  - Kuppusamy P
LA  - eng
GR  - R01 EB000890/EB/NIBIB NIH HHS/United States
GR  - R01 EB000890-04/EB/NIBIB NIH HHS/United States
GR  - CA 78886/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Validation Study
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Metalloporphyrins)
RN  - 0 (Molecular Probes)
RN  - 0 (lithium 5,9,14,18,23,27,32,36-octa-n-butoxy-2,3-naphthocyanine)
RN  - 723JX6CXY5 (Vitamin K 3)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Aorta/drug effects/metabolism
MH  - Cell Respiration/physiology
MH  - Dose-Response Relationship, Drug
MH  - Electron Spin Resonance Spectroscopy/*instrumentation/methods
MH  - Endothelium, Vascular/drug effects/*metabolism
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Lipopolysaccharides/pharmacology
MH  - *Metalloporphyrins
MH  - Mice
MH  - Microchemistry/*instrumentation/methods
MH  - Molecular Probes
MH  - Oximetry/*instrumentation/methods
MH  - Oxygen/*analysis/*metabolism
MH  - Oxygen Consumption/drug effects/*physiology
MH  - Sensitivity and Specificity
MH  - Vitamin K 3/pharmacology
EDAT- 2003/11/19 05:00
MHDA- 2004/02/14 05:00
CRDT- 2003/11/19 05:00
PHST- 2003/11/19 05:00 [pubmed]
PHST- 2004/02/14 05:00 [medline]
PHST- 2003/11/19 05:00 [entrez]
AID - S0003986103004764 [pii]
AID - 10.1016/j.abb.2003.09.008 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2003 Dec 1;420(1):169-75. doi: 10.1016/j.abb.2003.09.008.