PMID- 14624457
OWN - NLM
STAT- MEDLINE
DCOM- 20040720
LR  - 20091119
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 90
IP  - 5
DP  - 2003 Dec 1
TI  - Analysis of the mechanism regulating the stability of rat macrophage inflammatory 
      protein-2 mRNA in RBL-2H3 cells.
PG  - 976-86
AB  - Using rat peritoneal neutrophils, the complete nucleotide sequence of rat 
      macrophage inflammatory protein-2 (MIP-2) mRNA including 5'untranslated region 
      (UTR) and 3'UTR was determined (GenBank Accession number, AB060092). It was found 
      that the MIP-2 mRNA has a 70 bp 5'UTR, a 303 bp coding region and a 728 bp 3'UTR 
      which contains adenylate/uridylate (AU)-rich areas defined as AU-rich elements 
      (AREs). Site-directed mutagenesis studies using the tetracycline-sensitive 
      transactivator protein-expressing rat basophilic leukemia cells (RBL-2H3-TO 
      cells) revealed that MIP-2 mRNA mutants which lack the 3'UTR are more stable than 
      MIP-2-wild-type (wt) mRNA. A MIP-2 mRNA mutant in which some mutations were 
      introduced to the ARE was also stable. The stability of MIP-2 mRNA was low in 
      untreated RBL-2H3-TO cells, but it increased in the antigen-stimulated 
      immunoglobulin E (IgE)-sensitized cells. The antigen-induced MIP-2 mRNA 
      stabilization was counteracted by the highly specific p38 mitogen-activated 
      protein kinase (MAPK) inhibitor SB203580 and the MAPK/ERK kinase (MEK-1) 
      inhibitor PD98059. These findings indicate that ARE is the cis-element which 
      mediates the rapid decay of MIP-2 mRNA, and the antigen stimulation stabilizes 
      MIP-2 mRNA and the p38 MAPK and p44/42 MAPK pathways are involved in the 
      antigen-induced stabilization of MIP-2 mRNA.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Numahata, Keiichi
AU  - Numahata K
AD  - Laboratory of Pathophysiological Biochemistry, Graduate School of Pharmaceutical 
      Sciences, Tohoku University, Aoba Aramaki, Aoba-ku, Sendai, Miyagi 980-8578, 
      Japan.
FAU - Komagata, Tatsuya
AU  - Komagata T
FAU - Hirasawa, Noriyasu
AU  - Hirasawa N
FAU - Someya, Koh-ichiro
AU  - Someya K
FAU - Xiao, Yi-Qun
AU  - Xiao YQ
FAU - Ohuchi, Kazuo
AU  - Ohuchi K
LA  - eng
SI  - GENBANK/AB060092
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Monokines)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 1)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - 5' Untranslated Regions/genetics
MH  - Animals
MH  - Base Sequence
MH  - Chemokine CXCL2
MH  - Enzyme Inhibitors/pharmacology
MH  - Leukemia/*genetics/metabolism/pathology
MH  - MAP Kinase Kinase 1
MH  - Male
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors/metabolism
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Molecular Sequence Data
MH  - Monokines/*genetics/metabolism
MH  - Mutagenesis, Site-Directed
MH  - Neutrophils/drug effects/enzymology
MH  - Peritoneum/cytology
MH  - *RNA Stability
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Regulatory Sequences, Nucleic Acid/genetics
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2003/11/19 05:00
MHDA- 2004/07/21 05:00
CRDT- 2003/11/19 05:00
PHST- 2003/11/19 05:00 [pubmed]
PHST- 2004/07/21 05:00 [medline]
PHST- 2003/11/19 05:00 [entrez]
AID - 10.1002/jcb.10710 [doi]
PST - ppublish
SO  - J Cell Biochem. 2003 Dec 1;90(5):976-86. doi: 10.1002/jcb.10710.