PMID- 14624526
OWN - NLM
STAT- MEDLINE
DCOM- 20040803
LR  - 20181130
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 67
IP  - 4
DP  - 2003 Dec 15
TI  - Fabrication and in vitro testing of polymeric delivery system for condensed DNA.
PG  - 1384-92
AB  - Polyethylenimine (PEI) was combined with plasmid DNA and freeze dried following 
      the addition of sucrose as a lyoprotectant and pore-forming agent. Freeze-dried 
      PEI DNA condensates were dry mixed with granular polylactideglycolic acid (PLGA) 
      then compression molded and sponged to encapsulated PEI DNA. A measurement of the 
      elastic modulus indicated that 91 wt% sucrose substituted for 95 wt% sodium 
      chloride as a porogen, resulting in PLGA sponges with a mechanical modulus of 100 
      kPa. The PEI DNA was retained (80%) within PLGA sponges prepared with sucrose 
      during the leaching and subsequent 2-week release studies, whereas sodium 
      chloride PLGA sponges caused the premature release (100%) of PEI DNA within 2 
      days. In vitro gene transfer studies with PEI DNA PLGA sponges established that 
      adherent and infiltrating fibroblasts expressed reporter gene for 15 days 
      compared with the short, 3-day expression mediated by direct gene of PEI DNA on 
      cells in culture. The results demonstrate an approach to encapsulate condensed 
      DNA in a PLGA sponge for the purpose of retaining DNA within the matrices and 
      creating efficient gene transfer during tissue engineering.
CI  - Copyright 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1384-1392, 2003
FAU - Huang, Yen-Chen
AU  - Huang YC
AD  - College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065, USA.
FAU - Connell, Maureen
AU  - Connell M
FAU - Park, Youmie
AU  - Park Y
FAU - Mooney, David J
AU  - Mooney DJ
FAU - Rice, Kevin G
AU  - Rice KG
LA  - eng
GR  - DE13004/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (DNA, Circular)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 57-50-1 (Sucrose)
RN  - 9002-98-6 (Polyethyleneimine)
SB  - IM
MH  - Animals
MH  - DNA, Circular/*chemistry/metabolism
MH  - Elasticity
MH  - Fibroblasts/cytology/physiology
MH  - Gene Expression Regulation
MH  - *Gene Transfer Techniques
MH  - Genes, Reporter
MH  - Lactic Acid/*chemistry
MH  - Materials Testing
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Particle Size
MH  - Plasmids/*chemistry/metabolism
MH  - Polyethyleneimine/*chemistry
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
MH  - Sucrose/chemistry
MH  - Surface Properties
EDAT- 2003/11/19 05:00
MHDA- 2004/08/04 05:00
CRDT- 2003/11/19 05:00
PHST- 2003/11/19 05:00 [pubmed]
PHST- 2004/08/04 05:00 [medline]
PHST- 2003/11/19 05:00 [entrez]
AID - 10.1002/jbm.a.20036 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2003 Dec 15;67(4):1384-92. doi: 10.1002/jbm.a.20036.