PMID- 14626625
OWN - NLM
STAT- MEDLINE
DCOM- 20031202
LR  - 20211203
IS  - 0300-9742 (Print)
IS  - 0300-9742 (Linking)
VI  - 32
IP  - 4
DP  - 2003
TI  - High diagnostic performance of ELISA detection of antibodies to citrullinated 
      antigens in rheumatoid arthritis.
PG  - 197-204
AB  - OBJECTIVE: We investigated the rheumatoid arthritis (RA) diagnostic performances 
      of anti-cyclic citrullinated peptide antibody (anti-CCP) and antifilaggrin 
      antibody (AFA) in comparison with RF and matrix metalloproteinase-3 (MMP-3). 
      METHODS: We used a second generation enzyme-linked immunosorbent assay (ELISA) 
      kit for the detection of anti-CCP. We constructed recombinant human filaggrin, 
      which was citrullinated in vitro by human peptidylarginine deiminase, and 
      subsequently used it as the coating antigen for AFA-ELISA. A total of 549 RA 
      patients and 208 other rheumatic disease patients were included in the study. 
      RESULTS: The specificities of anti-CCP (88.9%) and AFA (94.7%) were superior to 
      those of RF (81.7%) and MMP-3 (49.5%). The sensitivity of anti-CCP (87.6%) was 
      superior to all others. However, the sensitivity of AFA (68.7%) was inferior to 
      those of RF (69.8%) and MMP-3 (75.7%). Furthermore, receiver operating 
      characteristic curves of anti-CCP and AFA passed closer to the upper left corner 
      than those of RF and MMP-3, and the areas under the curves (AUC) of AFA and 
      anti-CCP were significantly larger. In addition, the AUC of anti-CCP was 
      significantly larger than that of AFA. CONCLUSION: ELISA detection of antibodies 
      to citrullinated antigens, especially a second generation anti-CCP, showed higher 
      discriminative ability than other assays, including RF, and would be useful to 
      aid the diagnosis of RA in clinical practice.
FAU - Suzuki, K
AU  - Suzuki K
AD  - Department of Allergy and Rheumatology, University of Tokyo School of Medicine, 
      Tokyo, Japan
FAU - Sawada, T
AU  - Sawada T
FAU - Murakami, A
AU  - Murakami A
FAU - Matsui, T
AU  - Matsui T
FAU - Tohma, S
AU  - Tohma S
FAU - Nakazono, K
AU  - Nakazono K
FAU - Takemura, M
AU  - Takemura M
FAU - Takasaki, Y
AU  - Takasaki Y
FAU - Mimori, T
AU  - Mimori T
FAU - Yamamoto, K
AU  - Yamamoto K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Scand J Rheumatol
JT  - Scandinavian journal of rheumatology
JID - 0321213
RN  - 0 (Autoantibodies)
RN  - 0 (DNA Primers)
RN  - 0 (FLG protein, human)
RN  - 0 (Filaggrin Proteins)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (Recombinant Proteins)
RN  - 29VT07BGDA (Citrulline)
RN  - 9009-79-4 (Rheumatoid Factor)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Area Under Curve
MH  - Arthritis, Rheumatoid/blood/*diagnosis/immunology
MH  - Autoantibodies/*blood
MH  - Citrulline/*immunology
MH  - DNA Primers/chemistry
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Filaggrin Proteins
MH  - Humans
MH  - Intermediate Filament Proteins/immunology
MH  - Male
MH  - Matrix Metalloproteinase 3/blood
MH  - Middle Aged
MH  - Peptides, Cyclic/immunology
MH  - ROC Curve
MH  - Recombinant Proteins/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rheumatoid Factor/blood
MH  - Sensitivity and Specificity
EDAT- 2003/11/25 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/11/25 05:00
PHST- 2003/11/25 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/11/25 05:00 [entrez]
AID - 10.1080/03009740310003677 [doi]
PST - ppublish
SO  - Scand J Rheumatol. 2003;32(4):197-204. doi: 10.1080/03009740310003677.