PMID- 14627157
OWN - NLM
STAT- MEDLINE
DCOM- 20031202
LR  - 20081121
IS  - 0022-3395 (Print)
IS  - 0022-3395 (Linking)
VI  - 89
IP  - 5
DP  - 2003 Oct
TI  - Cloning of Schistosoma mansoni sporocysts in vitro and detection of genetic 
      heterogeneity among individuals within clones.
PG  - 1056-60
AB  - The establishment of in vitro cultivation techniques to maintain larval and adult 
      stages of the trematode Schistosoma mansoni has facilitated research on diverse 
      aspects of the biology of this parasite. Because of the difficulty in obtaining 
      defined intramolluscan stages of this parasite, one aim of this study was to 
      develop an in vitro technique for the generation of defined clonal daughter 
      sporocyst (DSp) generations that originate from a single mother sporocyst. 
      Sporocysts died when cultured singly; however, when single sporocysts were 
      cultured in inserts within wells with about 1,000 others, the single individuals 
      produced daughters asexually. In recent years, evidence has been accumulating for 
      variability among, and within, schistosome populations. Such variability has been 
      seen in both larval and adult stages. Even within clonal cercariae, genomic and 
      biochemical heterogeneity has been observed, indicating the existence of a yet 
      unknown mechanism that generates variability during larval development. 
      Therefore, another aim of this study was to examine clonal DSps generated in 
      vitro for diversity regarding the presence or absence of a specific repetitive 
      DNA element (W1). Such sporocysts were found by molecular analysis to be 
      heterogeneous with respect to the occurrence of W1. This phenomenon had 
      previously been observed in clonal schistosome populations and described as 
      genomic instability. In this study, we provide the first molecular evidence that 
      variability can be generated within sporocyst generations, supporting the 
      hypothesis of mitotic recombination events during the asexual life stage of 
      schistosomes.
FAU - Bayne, C J
AU  - Bayne CJ
AD  - Department of Zoology, Oregon State University, Corvallis, Oregon 97331, USA. 
      baynec@science.oregonstate.edu
FAU - Grevelding, C G
AU  - Grevelding CG
LA  - eng
GR  - AI-16137/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Parasitol
JT  - The Journal of parasitology
JID - 7803124
SB  - IM
MH  - Animals
MH  - Biomphalaria
MH  - Cloning, Molecular
MH  - Coculture Techniques
MH  - Cricetinae
MH  - Female
MH  - *Genetic Variation
MH  - Male
MH  - Mesocricetus
MH  - Schistosoma mansoni/*genetics/growth & development
EDAT- 2003/11/25 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/11/25 05:00
PHST- 2003/11/25 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/11/25 05:00 [entrez]
AID - 10.1645/GE-3186RN [doi]
PST - ppublish
SO  - J Parasitol. 2003 Oct;89(5):1056-60. doi: 10.1645/GE-3186RN.