PMID- 14627957
OWN - NLM
STAT- MEDLINE
DCOM- 20031215
LR  - 20171116
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 9
DP  - 2003 Nov 3
TI  - Epithelial activity of hexokinase and glucose-6-phosphate dehydrogenase in 
      cultured bovine lenses recovering from pharmaceutical-induced optical damage.
PG  - 594-600
AB  - PURPOSE: In a previous toxicological study, cultured bovine lenses exposed to 
      three topical anesthetics displayed distinct patterns of optical damage and 
      recovery. This work investigated the epithelial activity of the metabolic enzymes 
      hexokinase (HK) and glucose-6-phosphate dehydrogenase (G6PD) in lenses recovering 
      from anesthetic-induced damage. METHODS: Cultured bovine lenses were exposed to 
      the anesthetics Alcaine, Fluress and Fluoracaine for 2 h. An automated laser 
      scanner was used to determine the focal length variability (FLV) of the lenses at 
      time-points up to 24 h following their return to fresh culture medium. The 
      epithelial enzyme activities for HK and G6PD were then assayed at the 24 h 
      time-point. RESULTS: Lenses exposed to Alcaine displayed an abrupt increase in 
      FLV, while Fluoracaine treated lenses exhibited optical damage at a slower rate. 
      The FLV in these two groups recovered to near-control levels after 24 h. Fluress 
      treated lenses did not differ in FLV from controls at any time. The activities of 
      both HK and G6PD were significantly reduced in epithelial samples from each of 
      the three anesthetic treatment groups, relative to controls. CONCLUSIONS: These 
      results show that lens optical quality can recover despite a severe reduction in 
      epithelial HK and G6PD activity, indicating that the optical function of the lens 
      may not be directly related to epithelial metabolic activity. The ScanTox In 
      Vitro Assay System provides an objective measure of lens optical quality, 
      enabling a direct comparison of optical damage and recovery to lens biochemical 
      changes.
FAU - Hartwick, Andrew T E
AU  - Hartwick AT
AD  - School of Optometry, University of Waterloo, Waterloo, Ontario, Canada.
FAU - Sivak, Jacob G
AU  - Sivak JG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031103
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Anesthetics, Local)
RN  - 0 (Culture Media)
RN  - 0 (Drug Combinations)
RN  - 0 (Fluoresceins)
RN  - 0 (Preservatives, Pharmaceutical)
RN  - 37209-61-3 (Fluress)
RN  - 4Z8Y51M438 (Procaine)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - B4OB0JHI1X (proxymetacaine)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EPD1EH7F53 (Propoxycaine)
RN  - FZ989GH94E (Povidone)
RN  - HM4YQM8WRC (Chlorobutanol)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Anesthetics, Local/*toxicity
MH  - Animals
MH  - Cattle
MH  - Chlorobutanol/toxicity
MH  - Culture Media
MH  - Drug Combinations
MH  - Edetic Acid/toxicity
MH  - Epithelial Cells/*enzymology
MH  - Fluorescein
MH  - Fluoresceins/toxicity
MH  - Glucosephosphate Dehydrogenase/*metabolism
MH  - Hexokinase/*metabolism
MH  - Lens, Crystalline/cytology/*drug effects/*physiology
MH  - Organ Culture Techniques
MH  - Povidone/toxicity
MH  - Preservatives, Pharmaceutical
MH  - Procaine/*analogs & derivatives/toxicity
MH  - Propoxycaine/toxicity
EDAT- 2003/11/25 05:00
MHDA- 2003/12/16 05:00
CRDT- 2003/11/25 05:00
PHST- 2003/11/25 05:00 [pubmed]
PHST- 2003/12/16 05:00 [medline]
PHST- 2003/11/25 05:00 [entrez]
AID - v9/a72 [pii]
PST - epublish
SO  - Mol Vis. 2003 Nov 3;9:594-600.