PMID- 14628733
OWN - NLM
STAT- MEDLINE
DCOM- 20040305
LR  - 20191210
IS  - 0024-7758 (Print)
IS  - 0024-7758 (Linking)
VI  - 48
IP  - 10
DP  - 2003 Oct
TI  - Evaluation of sex chromosome aneuploidies in women with Turner's syndrome by 
      G-banding and FISH. A serial case study.
PG  - 804-8
AB  - OBJECTIVE: To evaluate sex chromosome aneuploidies in patients with Turner's 
      syndrome using two cytogenetic techniques. STUDY DESIGN: A sample of 35 women 
      with a clinical suspicion of Turner syndrome was examined in the Hospital of 
      Obstetrics and Gynecology, Instituto Mexicano del Seguro Social, Monterrey, 
      Mexico. They were subjected to a conventional cytogenetic technique with 
      G-banding and to fluorescence in situ hybridization (FISH) using a specific alpha 
      satellite X chromosome (DXZ1) and specific alpha satellite Y chromosome (DYZ1). 
      RESULTS: Using both techniques, 17 cases (48.57%) showed the same karyotype. 
      Using FISH: (1) in 8 cases the presence of the Y chromosome was confirmed, (2) in 
      18 cases (51.43%) a new cell line was identified, (3) in 2 cases (5.71%) the 
      derivative X was clarified, and (4) in 3 cases (8.57%) the origin of the 
      chromosome markers (1 of X chromosome and 2 of Y chromosome) was delineated. FISH 
      highlighted the differences between the initial diagnosis, based on G-banding, 
      and the final diagnosis, determined by specific probes for the X and Y 
      chromosomes. CONCLUSION: FISH is a useful tool in the detection of low-frequency 
      cell lines and identification of the nature and origin of derivative chromosomes 
      and unknown chromosome markers that have important implications for the treatment 
      of patients with Turner's syndrome.
FAU - Cortes-Gutierrez, Elva I
AU  - Cortes-Gutierrez EI
AD  - Genetics Division, School of Nursing, Northeast Center for Biomedical Research, 
      Mexican Institute of Social Security, School of Biological Sciences, Ignacio 
      Morones Prieto, Nuevo Leon, Mexico. elvairenecortes@hotmail.com
FAU - Cerda-Flores, Ricardo M
AU  - Cerda-Flores RM
FAU - Silva-Cudish, Julie B
AU  - Silva-Cudish JB
FAU - Davila-Rodriguez, Martha I
AU  - Davila-Rodriguez MI
FAU - Hernandez-Herrera, Ricardo
AU  - Hernandez-Herrera R
FAU - Leal-Garza, Carlos H
AU  - Leal-Garza CH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - J Reprod Med
JT  - The Journal of reproductive medicine
JID - 0173343
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/methods
MH  - Predictive Value of Tests
MH  - Sex Chromosomes/genetics
MH  - Turner Syndrome/*genetics
EDAT- 2003/11/25 05:00
MHDA- 2004/03/06 05:00
CRDT- 2003/11/25 05:00
PHST- 2003/11/25 05:00 [pubmed]
PHST- 2004/03/06 05:00 [medline]
PHST- 2003/11/25 05:00 [entrez]
PST - ppublish
SO  - J Reprod Med. 2003 Oct;48(10):804-8.