PMID- 14629030
OWN - NLM
STAT- MEDLINE
DCOM- 20040416
LR  - 20190922
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 50
IP  - 3
DP  - 2003 Sep
TI  - Enhancement of immunogenicity of Jeg3 cells by ectopic expression of HLA-A*0201 
      and CD80.
PG  - 243-53
AB  - PROBLEM: The choriocarcinoma cell line Jeg3 suppresses immunity in vitro by 
      secretion of soluble factors like leukemia inhibitory factor suppressing 
      leukocyte activation. The cells lack expression of classical human leukocyte 
      antigen (HLA)-A and -B alleles but express some HLA-C, and non-classical HLA-G 
      and -E. Upon binding to killing inhibitory receptor on natural killer (NK) cells, 
      HLA-G prevents activation of cytolytic activity. We investigated whether Jeg3 
      cells are capable of immune stimulation after complementation with classical HLA 
      and T cell costimulatory signal CD80. METHOD OF STUDY: Jeg3 cells were transduced 
      to express HLA-A*0201 and/or CD80. Parental Jeg3 or transfectants Jeg3-A2, 
      Jeg3-CD80 or Jeg3-CD80-A2 were used to stimulate allogeneic resting and activated 
      peripheral blood lymphocytes (PBL). The different cell lines were loaded with a 
      HLA-A2-restricted Epstein-Barr virus (EBV) recall antigen peptide epitope and 
      antigen presenting ability was examined. T cell lines specific for Jeg3 and 
      transfectants were generated from HLA-A2 matched and nonmatched donors and 
      compared for expansion, phenotypes and cytolytic activity. RESULTS: While all 
      Jeg3 cell lines induced only marginal proliferation of resting T cells, 
      phytohemagglutinin (PHA)-activated T cells were stimulated by CD80 or CD80-A2 
      expressing Jeg3. Only the transfectant Jeg3-CD80-A2 was capable of specific T 
      cell stimulation by EBV recall antigen presentation. T cell lines of HLA-A2 
      non-matched donors stimulated with the Jeg3 transfectants showed significant 
      expansion only when HLA-A2 and the costimulus CD80 were present. T cells from 
      HLA-A2 positive donors did not expand significantly or differentially. No NK 
      cells grew under any condition. In Jeg3-CD80-A2 stimulated T cells lines CD8+ 
      cells expanded preferentially. These T cells exerted cytolytic activity toward 
      all Jeg3 cell lines. CONCLUSION: Our data suggest that, in spite of 
      immunosuppressive mechanisms, proliferative and cytolytic T cell responses are 
      induced by Jeg3 cells when classical HLA- and/or costimulatory signals are 
      present on the cells.
FAU - Koc, Serpil
AU  - Koc S
AD  - Abteilung fur Frauenheilkunde, Gynakologische Molekularbiologie, Frauenklinik, 
      FSU Jena, Jena, Germany.
FAU - Kather, Angela
AU  - Kather A
FAU - Markert, Udo R
AU  - Markert UR
FAU - Durst, Matthias
AU  - Durst M
FAU - Schneider, Achim
AU  - Schneider A
FAU - Kaufmann, Andreas M
AU  - Kaufmann AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (B7-1 Antigen)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
SB  - IM
MH  - Antigen-Presenting Cells/immunology/metabolism
MH  - B7-1 Antigen/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Female
MH  - HLA-A Antigens/genetics/*metabolism
MH  - HLA-A2 Antigen
MH  - Humans
MH  - Lymphocyte Activation/*immunology
MH  - T-Lymphocytes/*immunology/metabolism
MH  - Transduction, Genetic
EDAT- 2003/11/25 05:00
MHDA- 2004/04/17 05:00
CRDT- 2003/11/25 05:00
PHST- 2003/11/25 05:00 [pubmed]
PHST- 2004/04/17 05:00 [medline]
PHST- 2003/11/25 05:00 [entrez]
AID - 10.1034/j.1600-0897.2003.00072.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2003 Sep;50(3):243-53. doi: 
      10.1034/j.1600-0897.2003.00072.x.