PMID- 14629626
OWN - NLM
STAT- MEDLINE
DCOM- 20031210
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 58
IP  - 5
DP  - 2003 Nov
TI  - Uptake of Apoptotic K562 Leukaemia Cells by Immature Dendritic Cells is Greatly 
      Facilitated by Serum.
PG  - 541-9
AB  - Dendritic cells (DCs) are potent antigen-presenting cells and play an important 
      role in T-cell-mediated immunity. DCs have been shown to induce strong antitumour 
      immune responses both in vitro and in vivo. One way of providing the DCs with all 
      relevant tumour antigens would be to incubate the DCs with material from dead 
      tumour cells. We have examined the uptake of apoptotic and necrotic K562 
      leukaemia cells by DCs under different culture conditions. Results from 
      coincubation experiments strongly suggested that uptake of apoptotic K562 cells 
      was dependent upon the addition of autologous serum (AS). Under these conditions, 
      47-79% of all DCs were shown to ingest apoptotic material. AS also seemed to be 
      important for the expression of functionally important markers, most notably HLA 
      class I, CD86, CCR7 and CD83. The vast majority of DCs were shown to ingest 
      necrotic material from K562 cells, with no additional effect of AS. The results 
      suggest that incubation of DCs with apoptotic material for cell therapeutic 
      purposes may best be performed in the presence of AS.
FAU - Dalgaard, J
AU  - Dalgaard J
AD  - Institute of Immunology, Rikshospitalet University Hospital, Oslo, Norway. 
      jakob.dalgaard@labmed.uio.no
FAU - Beckstrom, K J
AU  - Beckstrom KJ
FAU - Brinchmann, J E
AU  - Brinchmann JE
LA  - eng
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
SB  - IM
MH  - *Apoptosis
MH  - Dendritic Cells/*immunology
MH  - Endocytosis
MH  - Humans
MH  - Immunophenotyping
MH  - K562 Cells/*pathology
MH  - Necrosis
MH  - Serum/*physiology
EDAT- 2003/11/25 05:00
MHDA- 2003/12/12 05:00
CRDT- 2003/11/25 05:00
PHST- 2003/11/25 05:00 [pubmed]
PHST- 2003/12/12 05:00 [medline]
PHST- 2003/11/25 05:00 [entrez]
AID - 1332 [pii]
AID - 10.1046/j.1365-3083.2003.01332.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2003 Nov;58(5):541-9. doi: 10.1046/j.1365-3083.2003.01332.x.