PMID- 14630123 OWN - NLM STAT- MEDLINE DCOM- 20040422 LR - 20121115 IS - 0043-1354 (Print) IS - 0043-1354 (Linking) VI - 38 IP - 1 DP - 2004 Jan TI - Enhancement of 2,4-dichlorophenol degradation in conventional activated sludge systems bioaugmented with mixed special culture. PG - 245-53 AB - Efficient and reliable removal of recalcitrant compounds, which are present in wastewater intermittently, is critically important to prevent toxicity discharge and system upset, especially for those biotreatment systems with poor anti-shock loading capacity such as conventional activated sludge (CAS) systems. In this study, 2,4-dichlorophenol (2,4-DCP) was chosen as a model recalcitrant substance. 2,4-DCP degrading mixed culture was bioaugmented to a CAS system in terms of enhancing 2,4-DCP removal and maintaining system stability under shock loading conditions. The effects of bioaugmentation on the performance of CAS systems after single inoculation were investigated under long-term continuous operation, during which four times shock loading occurred. Results showed that the two bioaugmented CAS systems, which were inoculated with 5% and 15% 2,4-DCP degrading mixed culture, respectively, demonstrated and maintained stronger ability to degrade 2,4-DCP than the non-supplemented control one during the first three shock loading periods within the first month after the first inoculation. For the fourth 2,4-DCP shock loading which occurred 100 days after inoculation, the advantages demonstrated by the bioaugmented systems were greatly reduced compared to those of the previous three runs. In addition, ribosomal intergenic spacer analysis method was used to track the supplemented special culture and assay the effect of bioaugmentation on the changes of microbial community structure. FAU - Quan, Xiangchun AU - Quan X AD - State Key Joint Laboratory of Environment Simulation and Pollution Control, Department of Environmental Science and Engineering, Beijing Normal University, 100875, Beijing, People's Republic of China. quanxiangchun@tsinghua.org.cn FAU - Shi, Hanchang AU - Shi H FAU - Liu, Hong AU - Liu H FAU - Lv, Pingping AU - Lv P FAU - Qian, Yi AU - Qian Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Water Res JT - Water research JID - 0105072 RN - 0 (Anthelmintics) RN - 0 (Chlorophenols) RN - 0 (Sewage) RN - R669TG1950 (2,4-dichlorophenol) SB - IM MH - Anthelmintics/*metabolism MH - Biodegradation, Environmental MH - Chlorophenols/*metabolism MH - *Models, Theoretical MH - Population Dynamics MH - Sewage/*chemistry/*microbiology MH - Waste Disposal, Fluid/*methods MH - Water Purification/*methods EDAT- 2003/11/25 05:00 MHDA- 2004/04/23 05:00 CRDT- 2003/11/25 05:00 PHST- 2003/11/25 05:00 [pubmed] PHST- 2004/04/23 05:00 [medline] PHST- 2003/11/25 05:00 [entrez] AID - S0043-1354(03)00503-7 [pii] AID - 10.1016/j.watres.2003.09.003 [doi] PST - ppublish SO - Water Res. 2004 Jan;38(1):245-53. doi: 10.1016/j.watres.2003.09.003.