PMID- 14632405
OWN - NLM
STAT- MEDLINE
DCOM- 20040203
LR  - 20191210
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 86
IP  - 5
DP  - 2003 Sep-Oct
TI  - Determination of fungicide residues in white grapes for winemaking by gas 
      chromatography with mass spectrometric detection and assessment of matrix 
      effects.
PG  - 1008-14
AB  - A new analytical method, based on organic solvent extraction with 
      dichloromethane-acetone (75 + 25, v/v) followed by gas chromatography with mass 
      spectrometric detection, is presented for the determination of residues of 10 
      fungicides in white grapes for vinification. Some of them (cyprodinil, 
      fludioxonil, and pyrimethanil) have been used for only 2-3 years and, therefore, 
      no methods are available in the scientific literature for such a screening. 
      Quality parameters yielded good precision (relative standard deviation of <10%) 
      and detection limits (ranging between 1 and 18 microg/kg) that are lower than the 
      maximum residue limits (MRLs) set by the 76/895/European Economic Community (EEC) 
      and 90/642/EEC Directives. The applicability of the method was evaluated by 
      analysis of 5 different white grapes produced in the Rias Baixas area in Galicia 
      (northwestern Spain) for vinification. The method showed good performance in 
      analyses of real samples to determine whether the concentrations of the 
      fungicides used exceeded their MRLs. The method of standard additions was found 
      to be necessary to avoid matrix effects in the quantification of fungicide 
      residues. Results showed that concentrations of the fungicides identified in 
      grapes were lower than the MRLs established by the European legislation.
FAU - Gonzalez, Carlos Fernandez
AU  - Gonzalez CF
AD  - University of Vigo, Faculty of Food Science and Technology, Analytical and Food 
      Chemistry Department, Nutrition and Bromatology Group, Ourense Campus, 32004 
      Ourense, Spain.
FAU - Otero, Raquel Rial
AU  - Otero RR
FAU - Grande, Beatriz Cancho
AU  - Grande BC
FAU - Gandara, Jesus Simal
AU  - Gandara JS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Fungicides, Industrial)
RN  - 0 (Solvents)
RN  - 1364PS73AF (Acetone)
RN  - 588X2YUY0A (Methylene Chloride)
SB  - IM
MH  - Acetone
MH  - Drug Residues/*analysis
MH  - Fruit/*chemistry
MH  - Fungicides, Industrial/*analysis
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Methylene Chloride
MH  - Sensitivity and Specificity
MH  - Solvents
MH  - Spain
MH  - Vitis/*chemistry
MH  - *Wine/analysis
EDAT- 2003/11/25 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/11/25 05:00
PHST- 2003/11/25 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/11/25 05:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 2003 Sep-Oct;86(5):1008-14.