PMID- 14635227
OWN - NLM
STAT- MEDLINE
DCOM- 20040108
LR  - 20101118
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 74
IP  - 5
DP  - 2003 Dec 1
TI  - Hepatocyte growth factor stimulates cell motility in cultures of the striatal 
      progenitor cells ST14A.
PG  - 760-8
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a growth factor with 
      pleiotropic effects on different cell types. It acts as a mitogen and motility 
      factor for many epithelial cells. HGF/SF and its receptor Met are present in the 
      developing and adult mammalian brain and control neuritogenesis of sympathetic 
      and sensory neurons. We report that the striatal progenitor ST14A cells express 
      the Met receptor, which is activated after binding with HGF/SF. The interaction 
      between Met and HGF/SF triggers a signaling cascade that leads to increased 
      levels of c-Jun, c-Fos, and Egr-1 proteins, in agreement with data reported on 
      the signaling events evoked by HGF in other cellular types. We also studied the 
      effects of the exposure of ST14A cells to HGF/SF. By time-lapse photography, we 
      observed that a 24-hr treatment with 50 ng/ml HGF/SF induced modification in cell 
      morphology, with a decrease in cell-cell interactions and increase of cell 
      motility. In contrast, no effect on cell proliferation was observed. To 
      investigate which intracellular pathway is primarily involved we used PD98059 and 
      LY294002, two specific inhibitors of mitogen-activated protein 
      kinase/extracellular signal-regulated kinase (MAP-kinase/ERK-kinase) and 
      phosphoinositide 3-OH kinase (PI3-K), respectively. Cell motility in HGF/SF 
      treated cultures was inhibited by LY294002 but not by PD98059, suggesting that 
      PI3-K plays a key role in mediating the HGF/SF-induced dissociation of ST14A 
      cells. Previous evidence of HGF stimulation of motility in nervous system has 
      been obtained on postmitotic neurons, which have already acquired their 
      specificity. Data reported here of a motogenic response of ST14A cell line, which 
      displays properties of neuronal progenitors, seem of interest because they 
      suggest that HGF could play a role in very early steps of neurogenesis.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Cacci, E
AU  - Cacci E
AD  - Dipartimento di Biologia Cellulare e dello Sviluppo, Universita La Sapienza, 
      Roma, Italy.
FAU - Salani, M
AU  - Salani M
FAU - Anastasi, S
AU  - Anastasi S
FAU - Perroteau, I
AU  - Perroteau I
FAU - Poiana, G
AU  - Poiana G
FAU - Biagioni, S
AU  - Biagioni S
FAU - Augusti-Tocco, G
AU  - Augusti-Tocco G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Communication/drug effects
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cell Movement/*drug effects
MH  - Corpus Striatum/cytology/drug effects/embryology
MH  - Embryo, Mammalian
MH  - Genes, Immediate-Early/drug effects
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mitogen-Activated Protein Kinase Kinases/metabolism
MH  - Neurons/*drug effects/physiology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Precipitin Tests
MH  - Proto-Oncogene Proteins c-met/*physiology
MH  - Rats
MH  - Stem Cells/*drug effects/physiology
EDAT- 2003/11/25 05:00
MHDA- 2004/01/09 05:00
CRDT- 2003/11/25 05:00
PHST- 2003/11/25 05:00 [pubmed]
PHST- 2004/01/09 05:00 [medline]
PHST- 2003/11/25 05:00 [entrez]
AID - 10.1002/jnr.10799 [doi]
PST - ppublish
SO  - J Neurosci Res. 2003 Dec 1;74(5):760-8. doi: 10.1002/jnr.10799.