PMID- 146358
OWN - NLM
STAT- MEDLINE
DCOM- 19780321
LR  - 20191028
IS  - 0341-0382 (Print)
IS  - 0341-0382 (Linking)
VI  - 32
IP  - 11-12
DP  - 1977 Nov-Dec
TI  - Evidence for an 4-ene-3-oxosteroid-5alpha-reductase and delta4-delta5-ketosteroid 
      isomerase activity in extracts of Streptomyces hydrogenans.
PG  - 949-53
AB  - A homogenate of cells of Streptomyces hydrogenans which have been cultivated in 
      the presence of estradiol-17beta, was separated by gel filtration on Sephadex 
      G-200. In the presence of NADH, a few distinct fractions converted about 10% of 
      [3H]toesterone to 5alpha-dihydrotesterone (5alpha-DHT), 5alpha-androstanedione, 
      4-androstenedione, and dehydroepiandrosterone (DHEA). The metabolites were 
      separated after two consecutive runs on silica gel and 
      propanediol-1,2-impregnated cellulose plates by thin layer chromatography. 
      Identification was achieved by comparison with known steroid samples, specific 
      staining procedures and by crystallization to constant specific radioactivity. 
      The 5alpha-reductase activity responsible for the formation of 5alpha-DHT and 
      5alpha-androstanedione in vitro required NADH as co-substrate and could only be 
      found after induction of the cells with estradiol-17beta in vivo. Within the same 
      chromatographic fractions, a delta4-delta5-ketosteroid isomerase activity can be 
      detected which catalyzes the reverse reaction from the 4-androstene testosterone 
      to the 5-anthrostene DHEA in vitro.
FAU - Tinschert, W
AU  - Tinschert W
FAU - Trager, L
AU  - Trager L
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Z Naturforsch C Biosci
JT  - Zeitschrift fur Naturforschung. Section C, Biosciences
JID - 7801143
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.3.99.5 (3-Oxo-5-alpha-Steroid 4-Dehydrogenase)
RN  - EC 5.- (Isomerases)
RN  - EC 5.3.3.- (Steroid Isomerases)
SB  - IM
MH  - 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/*metabolism
MH  - Cytosol/enzymology
MH  - Enzyme Induction/drug effects
MH  - Estradiol/pharmacology
MH  - Isomerases/*metabolism
MH  - Oxidoreductases/*metabolism
MH  - Steroid Isomerases/*metabolism
MH  - Streptomyces/drug effects/*enzymology
MH  - Testosterone/metabolism
EDAT- 1977/11/01 00:00
MHDA- 1977/11/01 00:01
CRDT- 1977/11/01 00:00
PHST- 1977/11/01 00:00 [pubmed]
PHST- 1977/11/01 00:01 [medline]
PHST- 1977/11/01 00:00 [entrez]
AID - 10.1515/znc-1977-11-1212 [doi]
PST - ppublish
SO  - Z Naturforsch C Biosci. 1977 Nov-Dec;32(11-12):949-53. doi: 
      10.1515/znc-1977-11-1212.