PMID- 14637037
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20190901
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 97
IP  - 1-2
DP  - 2003 Dec 2
TI  - Oral transmission of porcine reproductive and respiratory syndrome virus by 
      muscle of experimentally infected pigs.
PG  - 45-54
AB  - The current study was performed to determine if porcine reproductive and 
      respiratory syndrome virus (PRRSV) could be transmitted to pigs by feeding muscle 
      tissue obtained from recently infected pigs. Muscle obtained from pigs infected 
      with either a European strain (EU donor pigs) or American strain (US donor pigs) 
      of PRRSV was fed to PRRSV-free receiver pigs. The donor pigs were slaughtered 11 
      days post-infection (dpi). PRRSV was detected by conventional virus isolation in 
      muscle at 11 dpi from 7 of 12 EU donor pigs and 5 of 12 US donor pigs. In 
      contrast to conventional virus isolation, all muscle samples from infected pigs 
      were positive for viral nucleic acid by PCR, except for muscle from one animal 
      infected with the American strain of PRRSV. Five hundred grams of raw 
      semimembranosus muscle from each of the donor pigs was fed over a 2 days period 
      (250 g per day) to each of two receiver pigs (48 receiver pigs). The receiver 
      pigs were housed separately in five groups. One of the five groups was fed muscle 
      obtained from US donor pigs that was also spiked with the American strain of 
      PRRSV. Sentinel pigs were placed in-contact with the group of receiver pigs fed 
      spiked muscle. All receiver pigs became viraemic by 6 days post-feeding (dpf). 
      There was evidence of horizontal transmission with sentinel pigs, in-contact with 
      receiver pigs, becoming viraemic. The study demonstrates that PRRSV could be 
      infectious through the oral route via the feeding of meat obtained from recently 
      infected pigs.
FAU - van der Linden, I F A
AU  - van der Linden IF
AD  - Department of Infectious Diseases, Animal Sciences Group, Institute of Animal 
      Science and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. 
      inge.vanderlinden@wur.nl
FAU - van der Linde-Bril, E M
AU  - van der Linde-Bril EM
FAU - Voermans, J J M
AU  - Voermans JJ
FAU - van Rijn, P A
AU  - van Rijn PA
FAU - Pol, J M A
AU  - Pol JM
FAU - Martin, R
AU  - Martin R
FAU - Steverink, P J G M
AU  - Steverink PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animal Feed/virology
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Meat/virology
MH  - Muscle, Skeletal/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*transmission
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - RNA, Viral/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Spleen/virology
MH  - Swine
EDAT- 2003/11/26 05:00
MHDA- 2004/03/20 05:00
CRDT- 2003/11/26 05:00
PHST- 2003/11/26 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2003/11/26 05:00 [entrez]
AID - S0378113503002980 [pii]
AID - 10.1016/j.vetmic.2003.09.015 [doi]
PST - ppublish
SO  - Vet Microbiol. 2003 Dec 2;97(1-2):45-54. doi: 10.1016/j.vetmic.2003.09.015.