PMID- 14637306
OWN - NLM
STAT- MEDLINE
DCOM- 20040126
LR  - 20231213
IS  - 1569-9048 (Print)
IS  - 1569-9048 (Linking)
VI  - 139
IP  - 1
DP  - 2003 Dec 16
TI  - Connexin36 distribution in putative CO2-chemosensitive brainstem regions in rat.
PG  - 1-20
AB  - Recent work from our laboratory has demonstrated that the gap junction proteins 
      connexin26 (Cx26) and connexin32 (Cx32) are expressed in neurons in putative 
      CO2-chemosensitive brainstem regions in both neonatal and adult rats. Whether the 
      recently identified neuron-specific gap junction protein connexin36 (Cx36) is 
      also present in these brainstem regions remains to be determined. Therefore, in 
      the current experiments, immunoblot and immunohistochemical protocols were used 
      to investigate the regional distribution and cellular localization of Cx36 in 
      putative CO2-chemosensitive brainstem regions in both neonatal and adult rats. 
      Immunoblot analyses revealed Cx36 expression in putative CO2-chemosensitive 
      brainstem regions in each of the age groups examined, although both regional and 
      developmental differences in the relative expression levels were detected. 
      Immunohistochemical analyses confirmed Cx36 expression in neurons in each of the 
      putative CO2-chemosensitive brainstem regions and revealed both somal and 
      dendritic labeling patterns. These findings provide additional morphological 
      evidence supporting the potential for gap junctional communication in these 
      regions in both neonatal and adult rats. We propose that the gap junction protein 
      Cx36 also contributes to the neuroanatomical substrate for gap junctional 
      communication, which is hypothesized to play a role in central CO2 
      chemoreception.
FAU - Solomon, Irene C
AU  - Solomon IC
AD  - Department of Physiology and Biophysics, State University of New York at Stony 
      Brook, Basic Science Tower, Health Science Center, Level 6, Rm. 140, Stony Brook, 
      NY 11794-8661, USA. icsolomon@physiology.pnb.sunysb.edu
LA  - eng
GR  - HL63175/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Respir Physiol Neurobiol
JT  - Respiratory physiology & neurobiology
JID - 101140022
RN  - 0 (Connexins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Neurokinin-1)
RN  - 0 (carbon dioxide receptor)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Behavior, Animal
MH  - Blotting, Western/methods
MH  - Brain Stem/cytology/*metabolism
MH  - Connexins/*metabolism
MH  - Dendrites/metabolism
MH  - Immunohistochemistry/methods
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neurons/cytology/metabolism
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cell Surface/*metabolism
MH  - Receptors, Neurokinin-1/metabolism
MH  - Tryptophan Hydroxylase/metabolism
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - Gap Junction delta-2 Protein
EDAT- 2003/11/26 05:00
MHDA- 2004/01/27 05:00
CRDT- 2003/11/26 05:00
PHST- 2003/11/26 05:00 [pubmed]
PHST- 2004/01/27 05:00 [medline]
PHST- 2003/11/26 05:00 [entrez]
AID - S1569904803002295 [pii]
AID - 10.1016/j.resp.2003.09.004 [doi]
PST - ppublish
SO  - Respir Physiol Neurobiol. 2003 Dec 16;139(1):1-20. doi: 
      10.1016/j.resp.2003.09.004.