PMID- 14638431
OWN - NLM
STAT- MEDLINE
DCOM- 20040210
LR  - 20181130
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 228
IP  - 2
DP  - 2003 Nov 21
TI  - Comparative analysis of Borrelia isolates from southeastern USA based on randomly 
      amplified polymorphic DNA fingerprint and 16S ribosomal gene sequence analyses.
PG  - 249-57
AB  - Fifty-three southern USA Borrelia isolates were characterized using randomly 
      amplified polymorphic DNA fingerprinting analysis (RAPD). Twenty-nine types were 
      recognized among 37 B. andersonii strains, seven types among eight B. bissettii 
      strains, and seven types among seven B. burgdorferi sensu stricto strains. Strain 
      TXW-1 formed a separate RAPD type. Nearly complete sequences of the rrs genes 
      from 17 representative southern Borrelia were determined. The similarity values 
      were found to be 96-100% within the B. burgdorferi sensu lato (s.l.) complex, 
      94-99% among the relapsing fever borreliae, and 93-99% between the two complexes. 
      Phylogenetic analysis indicated that all the Borrelia strains we analyzed could 
      be divided into two parts: the B. burgdorferi s.l. complex and the relapsing 
      fever borreliae complex. TXW-1 segregated with the North American relapsing fever 
      borreliae and formed a separate subbranch.
FAU - Lin, Tao
AU  - Lin T
AD  - Institute of Arthropodology and Parasitology, PO Box 8056, Georgia Southern 
      University, Statesboro, GA 30460-8056, USA.
FAU - Oliver, James H Jr
AU  - Oliver JH Jr
FAU - Gao, Lihui
AU  - Gao L
LA  - eng
GR  - R37 AI-24899/AI/NIAID NIH HHS/United States
GR  - U50/CCU410282/CC/ODCDC CDC HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Arachnid Vectors/microbiology
MH  - Borrelia/*classification/genetics/growth & development/isolation & purification
MH  - Borrelia burgdorferi/classification/genetics/isolation & purification
MH  - Borrelia burgdorferi Group/*classification/genetics/growth & 
      development/isolation & purification
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/analysis/isolation & purification
MH  - DNA, Ribosomal/analysis/isolation & purification
MH  - Disease Reservoirs
MH  - Genes, rRNA
MH  - Genotype
MH  - Lyme Disease/microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Random Amplified Polymorphic DNA Technique
MH  - Relapsing Fever/microbiology
MH  - Sequence Analysis, DNA
MH  - Southeastern United States
MH  - Ticks/microbiology
EDAT- 2003/11/26 05:00
MHDA- 2004/02/11 05:00
CRDT- 2003/11/26 05:00
PHST- 2003/11/26 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2003/11/26 05:00 [entrez]
AID - S0378109703007638 [pii]
AID - 10.1016/S0378-1097(03)00763-8 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2003 Nov 21;228(2):249-57. doi: 
      10.1016/S0378-1097(03)00763-8.