PMID- 14638736
OWN - NLM
STAT- MEDLINE
DCOM- 20031209
LR  - 20190607
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 44
IP  - 12
DP  - 2003 Dec
TI  - c-Jun expression in surviving and regenerating retinal ganglion cells: effects of 
      intravitreal neurotrophic supply.
PG  - 5342-8
AB  - PURPOSE: To investigate c-jun expression in surviving and axon-regenerating 
      retinal ganglion cells (RGCs) and the effect of intravitreal neurotrophic supply 
      on c-jun expression. METHODS: All animals underwent optic nerve transection (ONT) 
      0.5 mm behind the eyeball. Some animals underwent a replacement of the optic 
      nerve with an autologous sciatic nerve graft (SNG) to allow axonal regrowth. To 
      provide a neurotrophic supply, a peripheral nerve (PN) segment or brain-derived 
      neurotrophic factor (BDNF)/ciliary neurotrophic factor (CNTF) was applied 
      intravitreally. The time course of c-jun expression was first examined in both 
      surviving and regenerating RGCs. Then, c-jun expression was examined in surviving 
      and regenerating RGCs 3 weeks after intravitreal BDNF/CNTF treatment. Animals 
      with vehicle eye injection were used as the control. Fluorescent dye was used for 
      retrograde labeling of surviving (applied behind the eyeball) and regenerating 
      (applied at the distal end of the SNG) RGCs. All retinas were 
      immunohistochemically stained for c-jun. RESULTS: c-Jun was not detected in 
      normal RGCs, but weak expression was seen in surviving RGCs after ON injury. The 
      proportion of c-jun-positive (+) RGCs among surviving cell population was 52.6% 
      to 86.5% 2 to 6 weeks after ONT. Among regenerating RGCs, more than 80% expressed 
      c-jun in all treatment groups, a proportion that was significantly higher after 
      CNTF treatment (90.7%). In addition, c-jun expression was much stronger in 
      intensity and the c-jun(+) nuclei were much larger in regenerating than in 
      surviving RGCs. CONCLUSIONS: c-Jun expression in RGCs was upregulated after 
      injury. Most regenerating RGCs were c-jun(+), and the intensity of c-jun 
      expression was higher in regenerating than in surviving RGCs. CNTF also 
      upregulated c-jun expression in RGCs.
FAU - Lu, Qiang
AU  - Lu Q
AD  - Department of Anatomy, Faculty of Medicine, The University of Hong Kong, 21 
      Sassoon Road, Hong Kong, China.
FAU - Cui, Qi
AU  - Cui Q
FAU - Yip, Henry K
AU  - Yip HK
FAU - So, Kwok-Fai
AU  - So KF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Proto-Oncogene Proteins c-jun)
SB  - IM
MH  - Animals
MH  - Axons/physiology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Survival/physiology
MH  - Ciliary Neurotrophic Factor/*pharmacology
MH  - Cricetinae
MH  - Fluorescent Antibody Technique, Indirect
MH  - Injections
MH  - Mesocricetus
MH  - Optic Nerve Injuries/metabolism
MH  - Proto-Oncogene Proteins c-jun/*metabolism
MH  - Regeneration/physiology
MH  - Retinal Ganglion Cells/drug effects/*metabolism
MH  - Time Factors
MH  - Up-Regulation
MH  - Vitreous Body
EDAT- 2003/11/26 05:00
MHDA- 2003/12/11 05:00
CRDT- 2003/11/26 05:00
PHST- 2003/11/26 05:00 [pubmed]
PHST- 2003/12/11 05:00 [medline]
PHST- 2003/11/26 05:00 [entrez]
AID - 10.1167/iovs.03-0444 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2003 Dec;44(12):5342-8. doi: 10.1167/iovs.03-0444.