PMID- 14640976
OWN - NLM
STAT- MEDLINE
DCOM- 20040624
LR  - 20181113
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 378
IP  - Pt 3
DP  - 2004 Mar 15
TI  - The nuclear tRNA aminoacylation-dependent pathway may be the principal route used 
      to export tRNA from the nucleus in Saccharomyces cerevisiae.
PG  - 809-16
AB  - Nuclear tRNA export in Saccharomyces cerevisiae has been proposed to involve 
      three pathways, designated Los1p-dependent, Los1p-independent nuclear 
      aminoacylation-dependent, and Los1p- and nuclear aminoacylation-independent. 
      Here, a comprehensive biochemical analysis was performed to identify tRNAs 
      exported by the aminoacylation-dependent and -independent pathways of S. 
      cerevisiae. Interestingly, the major tRNA species of at least 19 families were 
      found in the aminoacylated form in the nucleus. tRNAs known to be exported by the 
      export receptor Los1p were also aminoacylated in the nucleus of both wild-type 
      and mutant Los1p strains. FISH (fluorescence in situ hybridization) analyses 
      showed that tRNA(Tyr) co-localizes with the U18 small nucleolar RNA in the 
      nucleolus of a tyrosyl-tRNA synthetase mutant strain defective in nuclear 
      tRNA(Tyr) export because of a block in nuclear tRNA(Tyr) aminoacylation. 
      tRNA(Tyr) was also found in the nucleolus of a utp8 mutant strain defective in 
      nuclear tRNA export but not nuclear tRNA aminoacylation. These results strongly 
      suggest that the nuclear aminoacylation-dependent pathway is principally 
      responsible for tRNA export in S. cerevisiae and that Los1p is an export receptor 
      of this pathway. It is also likely that in mammalian cells tRNAs are mainly 
      exported from the nucleus by the nuclear aminoacylation-dependent pathway. In 
      addition, the data are consistent with the idea that nuclear aminoacylation is 
      used as a quality control mechanism for ensuring nuclear export of only mature 
      and functional tRNAs, and that this quality assurance step occurs in the 
      nucleolus.
FAU - Steiner-Mosonyi, Marta
AU  - Steiner-Mosonyi M
AD  - Guelph-Waterloo Center for Graduate Work in Chemistry and Biochemistry, 
      Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario 
      N1G 2W1, Canada.
FAU - Mangroo, Dev
AU  - Mangroo D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Los1 protein, S cerevisiae)
RN  - 0 (Nuclear Pore Complex Proteins)
RN  - 0 (RNA, Transfer, Amino Acyl)
RN  - 0 (RNA, Transfer, Tyr)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 9014-25-9 (RNA, Transfer)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - Cell Nucleolus/chemistry
MH  - Cell Nucleus/chemistry/*metabolism
MH  - Mutation
MH  - Nuclear Pore Complex Proteins/metabolism
MH  - RNA, Transfer/*metabolism
MH  - RNA, Transfer, Amino Acyl/analysis/*metabolism
MH  - RNA, Transfer, Tyr/analysis
MH  - Saccharomyces cerevisiae/chemistry/genetics/*metabolism
MH  - Saccharomyces cerevisiae Proteins/metabolism
MH  - Signal Transduction
PMC - PMC1224000
EDAT- 2003/12/03 05:00
MHDA- 2004/06/25 05:00
PMCR- 2004/09/15
CRDT- 2003/12/03 05:00
PHST- 2003/11/28 00:00 [accepted]
PHST- 2003/11/05 00:00 [revised]
PHST- 2003/08/26 00:00 [received]
PHST- 2003/12/03 05:00 [pubmed]
PHST- 2004/06/25 05:00 [medline]
PHST- 2003/12/03 05:00 [entrez]
PHST- 2004/09/15 00:00 [pmc-release]
AID - BJ20031306 [pii]
AID - 10.1042/BJ20031306 [doi]
PST - ppublish
SO  - Biochem J. 2004 Mar 15;378(Pt 3):809-16. doi: 10.1042/BJ20031306.